Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nerve regeneration biogum and preparation method and application thereof

A technology of nerve regeneration and biological glue, which is applied in the field of biological tissue engineering and medical materials, to achieve the effect of increasing the connection between damaged and normal nerve synapses, promoting proliferation, and blocking inhibitory factors

Inactive Publication Date: 2013-06-05
SHANGHAI SHENYIN BIOTECH
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that pluripotent NSCs were isolated from the nervous tissue of animal embryos and successfully transplanted into spinal cord injury, brain tissue and optic nerve injury, and it was observed that they could survive, migrate and differentiate into neurons in the host; However, under this condition, most of the transplanted NSCs differentiated into glial cells, only a few differentiated into neurons, and the differentiated neurons grew to the extramedullary and could not achieve long-distance extension in the medulla and synapse with recipient neurons. tactile connections, the reason for which is related to neuronal regeneration after spinal cord injury and the local microenvironment of intramedullary extension

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nerve regeneration biogum and preparation method and application thereof
  • Nerve regeneration biogum and preparation method and application thereof
  • Nerve regeneration biogum and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation of the nerve regeneration biological glue of embodiment 1 low concentration

[0024] Add NaCl to deionized water to make 10ml of 0.8% (w / v) NaCl solution, filter through the filter head, dissolve 50mg of fibrinogen in it, add the neurotrophic factors shown in the following table 2 in turn, and finally add thrombin 25u, that is, the nerve regeneration biological glue of the present invention is obtained, wherein the final concentration of fibrinogen is 5mg / ml, and the final concentration of thrombin is 2.5u / ml. The bioglue is formed about 2 hours after preparation, has low viscosity and slightly low mechanical strength, and is suitable for in vitro cell culture.

[0025] Table 2

[0026] serial number

Embodiment 2

[0027] The preparation of the nerve regeneration biological glue of embodiment 2 high concentration

[0028] Add NaCl to deionized water to make 10ml of 0.8% (w / v) NaCl solution, filter it through a filter head, dissolve 400mg of fibrinogen in it, add the neurotrophic factors shown in Table 3 in turn, and finally add 250u of thrombin , to obtain the nerve regeneration biological glue of the present invention, wherein the final concentration of fibrinogen is 40mg / ml, and the final concentration of thrombin is 25u / ml. The bioglue is formed about 15 minutes after preparation, has high viscosity, high mechanical strength, and large porosity, and is easier to fix at the injection site, and is suitable for injection at the site of spinal cord injury in a living body (animal / human).

[0029] table 3

[0030] serial number

[0031] 9

Embodiment 3

[0032] Embodiment 3 in vitro cell test

[0033] 1. Preparation and culture of neural stem cells

[0034]14-16 day old SD rats were selected and sacrificed after intraperitoneal anesthesia with 10% chloral hydrate at 0.32ml / 100g body weight. Routine disinfection, laparotomy, removal of fetal mice, and placed in 4 ℃ PBS solution. Under sterile conditions, after the fetal brain was removed, the skull and meninges were peeled off, and the cortical tissue and hippocampal tissue were carefully separated under a dissecting microscope. Shred the tissue, digest with papain and DNase, pipette, filter, and form single cells, then inoculate and culture on a culture plate coated with Lamining, the medium is DMEM / F12, add cytokines B27, EGF, bFGF, LI for culture . Thereafter, the clones were mechanically separated and passaged every 5-7 days, and half of the medium was changed every 3-5 days according to the growth rate of the cells and the pH change of the culture medium. Continuously ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses nerve regeneration biogum and a preparation method and an application thereof. The nerve regeneration biogum is prepared from fibrinogen, NaCl, hemopexin and 12 neurotrophic factors, wherein the fibrinogen, the hemopexin and the NaCl form a base support matrix. The biogum disclosed by the invention can promote regeneration and intramedullary extension of a nerve cell, and can increase the contact frequency of damaged nerve cynapse and normal nerve synapse by verification of an in-vitro cell test and an animal test. A plurality of neurotrophic factors in the biogum have important roles in regeneration and extension of the nerve cell, building of synaptic connection and forming of a blood vessel, can be further used for clinically repairing spinal nerve trauma and treating patients with spinal cord injury. The nerve regeneration biogum can be gradually applied to other central nervous system diseases, such as injury of brain tissue, optic nerve injury and the like.

Description

technical field [0001] The invention belongs to the field of biological tissue engineering and medical materials, and relates to a kind of nerve regeneration bioglue and its preparation method and application, in particular to a kind of nerve regeneration bioglue containing a variety of neurotrophic factors and its preparation method and its application in nerve regeneration after spinal cord injury. Application in Restorative Therapy. Background technique [0002] Central nervous system injury, such as severe spinal cord injury, mainly manifests as paraplegia and quadriplegia. Studies have shown that synapses cannot be formed between neurons in the injured spinal cord to repair nerve conduction pathways. So far, there is no effective clinical treatment. . Studies have reported that intramedullary transplantation of adult stem cells, induced pluripotent stem cells, and embryonic stem cells can form new neurons in the spinal cord injury, thereby establishing synaptic connect...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K38/55A61K47/42A61P25/00A61K38/19A61K38/18A61K31/375
Inventor 朱剑虹朱侗明高华嵩
Owner SHANGHAI SHENYIN BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com