Growth-promoting bacteria for facilitating growth of rape and microbial organic fertilizer

A technology of organic fertilizer and growth-promoting bacteria, which is applied in the direction of organic fertilizer, organic fertilizer preparation, and microbial-based methods, can solve the problems of weakening the ability of sustainable agricultural development, loss of nutrient resources, and polluting the environment, so as to promote the growth of rapeseed. Growth, savings, high bioavailability effects

Active Publication Date: 2013-06-26
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0003] The incineration of crop straws in our country and the disposal of livestock and poultry manure after large-scale breeding not only seriously pollute the environment, but also greatly waste raw materials that can be used a

Method used

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  • Growth-promoting bacteria for facilitating growth of rape and microbial organic fertilizer
  • Growth-promoting bacteria for facilitating growth of rape and microbial organic fertilizer
  • Growth-promoting bacteria for facilitating growth of rape and microbial organic fertilizer

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Embodiment Construction

[0030] (1) Isolation of growth-promoting strains

[0031] Preliminary screening of growth-promoting strains: collect the rhizosphere soil of cruciferous plants from the Zijin Mountain forest soil, bathe in 80℃ water for 30 minutes, use the dilution coating technology to initially screen the bacillus, and obtain a total of 5 growth-promoting strains, and freeze-dry the strains Store at -20°C for further screening.

[0032] Re-screening of growth-promoting strains: Take the first-screened growth-promoting bacteria, culture them in NA liquid culture for 48 hours, centrifuge at 8000rpm / min, and resuspend in the same amount of sterile water to obtain ≥1×10 10 Cfu / ml bacterial suspension, half of the bacterial suspension was inactivated at 121°C for 20 minutes. The rapeseed with equivalent growth is treated as follows: Treatment 1: Soak the roots of the seedlings in clean water for 10 minutes; Treatment 2: Soak the roots of the seedlings in the bacterial suspension for 20 minutes at 121℃...

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Abstract

The invention relates to growth-promoting bacteria for facilitating growth of rape and microbial organic fertilizer, and belongs to the intensive agriculture production technique. The microbial organic fertilizer is prepared in the following steps of separating antagonistic bacteria NJZJSB 3, inoculating into a microbial decomposition mixture of thoroughly decomposed pig manure compost and rapeseed meal to carry out solid fermentation to obtain the microbial organic fertilizer, wherein the microbial organic fertilizer comprises more than 1*10<8>cfu/g growth-promoting bacteria, the content of full nitrogen is 4-5% (the content of organic nitrogen is more than 90%), the total nitrogen phosphorus and potassium nutrient is 6-10%, and the content of organic matters is not lower than 45%. Based on an experiment result, the fresh weights of ground parts, underground parts and the whole strain of rape are respectively up to 154.6%, 114.7% and 148.4% so that the growth of the rape can be effectively facilitated if the microbial organic fertilizer is applied in successive years.

Description

1. Technical Field [0001] The invention relates to a growth-promoting strain used to promote the growth of rapeseed and its microbial organic fertilizer, belonging to the technical field of agricultural intensive production. 2. Background technology [0002] Rape is an important oil crop in our country, and it plays an important role in the construction of the national economy. Promoting the growth of rapeseed and increasing the yield of rape are always important topics in the agricultural economy. In the process of rapeseed planting, due to the long-term investment of a large amount of chemical fertilizers, the soil environment where the rape grows is destroyed, the soil biological activity is severely reduced, and the number and number of beneficial microorganisms in the soil are reduced, resulting in the continuous decline of rape production. Therefore, the development of new fertilizers that promote the growth of rape is very important for the production of oil crops. [0003...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C05F17/00C12R1/07
CPCY02W30/40
Inventor 沈其荣黄启为吴云成凌宁
Owner NANJING AGRICULTURAL UNIVERSITY
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