Growth-promoting bacteria for facilitating growth of rape and microbial organic fertilizer
A technology of organic fertilizer and growth-promoting bacteria, which is applied in the direction of organic fertilizer, organic fertilizer preparation, and microbial-based methods, can solve the problems of weakening the ability of sustainable agricultural development, loss of nutrient resources, and polluting the environment, so as to promote the growth of rapeseed. Growth, savings, high bioavailability effects
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[0030] (1) Isolation of growth-promoting strains
[0031] Preliminary screening of growth-promoting strains: collect the rhizosphere soil of cruciferous plants from the Zijin Mountain forest soil, bathe in 80℃ water for 30 minutes, use the dilution coating technology to initially screen the bacillus, and obtain a total of 5 growth-promoting strains, and freeze-dry the strains Store at -20°C for further screening.
[0032] Re-screening of growth-promoting strains: Take the first-screened growth-promoting bacteria, culture them in NA liquid culture for 48 hours, centrifuge at 8000rpm / min, and resuspend in the same amount of sterile water to obtain ≥1×10 10 Cfu / ml bacterial suspension, half of the bacterial suspension was inactivated at 121°C for 20 minutes. The rapeseed with equivalent growth is treated as follows: Treatment 1: Soak the roots of the seedlings in clean water for 10 minutes; Treatment 2: Soak the roots of the seedlings in the bacterial suspension for 20 minutes at 121℃...
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