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Reagent device and method for detecting mycoplasma pneumonia antibody

A technology of mycoplasma pneumoniae and reagents, which is applied in the field of clinical immunology detection, and can solve the problems of inability to perform single-person detection, cumbersome reagent filling operations, and many types of reagent bottles

Active Publication Date: 2013-07-03
SHENZHEN YHLO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] (1) Use 12×8 type, 6×8 type, 8×12 type or full-plate type 96-well special microwell plate as antigen coating equipment and reaction container, which can only be divided into 12 batches and 6 batches when used , 8 batches or the whole board can be used at one time, and independent and single-person testing cannot be carried out;
[0014] (2) There are many kinds of reagents used in quantitative determination, and each detection reagent must be contained in a reagent bottle, and each time a reagent is used, the suction nozzle needs to be replaced to fill the microwells of the microwell plate respectively , not only there are many types of reagent bottles, but also the operation of filling reagents is extremely cumbersome;
[0015] (3) There is a lack of corresponding labeling of the testing information, and the production batch number and expiration date information of the testing reagent can only be known or known by checking the label on the outer packaging box of the kit, and the known information is not controlled during the testing process, which has great potential large randomness;
[0016] (4) The detection reagent is in an open space during the detection process, which may easily cause cross-contamination between various reagents and affect the accuracy of the detection result;
[0017] (5) Manual operation is mostly used in the detection process, the addition of reagents or samples is not very precise, the operation process is extremely cumbersome and complicated, and operation errors are prone to occur, and the accuracy and precision of the detection results are poor;
[0018] (6) The quantity configuration and use of the complete set of reagents for the test items are the number of items × 48 / 96 persons. If 10 items need to be tested, the configuration and use of the reagents must be 10 × 48 / 96 persons. If Only one sample needs to detect 10 different items, and it also needs to configure reagents for 10×48 / 96 people, which has the disadvantage of not being economical and reasonable

Method used

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  • Reagent device and method for detecting mycoplasma pneumonia antibody
  • Reagent device and method for detecting mycoplasma pneumonia antibody
  • Reagent device and method for detecting mycoplasma pneumonia antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Embodiment one detects the reagent device one of Mycoplasma pneumoniae antibody

[0095] Such as Figure 1-2 As shown, the reagent device for detecting Mycoplasma pneumoniae antibody described in the application of the present invention is in the shape of a strip, including a base body 10 with eight holes and a handle 20 positioned at one end of the base body 10, and the arrangement order of the eight holes starts from near the handle 10. Starting from the end, there are sample wells 11, auxiliary agent wells 12, enzyme conjugate wells 13, substrate wells 14, stop solution wells 15, diluent wells 16, reaction wells 17 and dilution wells 18. The sample wells 11 contain There is a sample to be tested, the auxiliary agent hole 12 is used for adding auxiliary reagents when the detection needs (such as adding an adsorbent to the auxiliary agent hole when detecting IgM), the enzyme conjugate solution is added to the enzyme conjugate hole 13, and the substrate is added to the ...

Embodiment 2

[0098] Example 2 Reagent Device 2 for Detecting Mycoplasma pneumoniae Antibody

[0099] Such as image 3 As shown, the reagent device for detecting Mycoplasma pneumoniae antibody in this embodiment has the same basic structure as that of the reagent device in Example 1, and the reagent device also includes several support columns 50, and the support columns 50 are located at the Under the substrate 10 in the above reagent device, there is more than one hole between adjacent supporting pillars 50. The function of the supporting pillars 50 is to enhance the mechanical strength and balance of the substrate.

Embodiment 3

[0100] Example 3 Reagent Device 3 for Detecting Mycoplasma pneumoniae Antibody

[0101] Such as Figure 4-6 As shown, it is a preferred embodiment of the reagent device for detecting Mycoplasma pneumoniae antibody described in the application of the present invention. Its basic structure is the same as that of Embodiment 1 or Embodiment 2, the difference is that the reaction hole 17 is a detachable structure, and the reaction hole 17 Consists of an outer hole 171 and an inner hole 172, the bottom of the outer hole 171 is provided with a bottom hole 174, the inner hole 172 passes through the bottom hole 174 and closely fits with the bottom hole 174, and there is an anti-overflow gap between the outer hole 171 and the inner hole 172 Cavity 173, the function of the anti-overflow cavity 173 is that during the reaction, if the liquid overflows, it can stay in the cavity to prevent contamination of instruments and other reagent devices.

[0102] Further, the cooperating fixing meth...

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Abstract

The invention provides a reagent device and a method used for detecting mycoplasma pneumonia antibodies. The reagent device has a long strip shape, and has a base body comprising 8 hole positions and a handle positioned on one end of the base body. From the end proximal to the handle, the 8 hole positions are sequentially a sample hole, an auxiliary agent hole, an enzyme conjugate hole, a substrate hole, a termination liquid hole, a dilution liquid hole, a reaction hole, and a dilution hole. According to the invention, based on a principle of enzyme-linked immunoassay, mycoplasma pneumonia antibody is detected by using the reagent device. The method is an independent single-person analysis and detection method. The device can be used in cooperation with a corresponding specific analysis instrument. During a detection process, detection reagents or samples are injected by using a full-automatic precise dosing device. The device and the method have the advantages of automatic operation, precise dosing, high detection result accuracy, high detection result precision, and wide application prospect.

Description

technical field [0001] The application of the present invention relates to a reagent device and a method for detecting mycoplasma pneumoniae antibody, belonging to the technical field of clinical immunology detection. Background technique [0002] The pathological changes of mycoplasma pneumonia are mainly interstitial pneumonia, sometimes complicated by bronchial pneumonia, which is called primary atypical pneumonia. It is mainly transmitted by droplets, with an incubation period of 2 to 3 weeks, and the highest incidence rate is among adolescents. The clinical symptoms are mild, or even asymptomatic. If there are, they only show general respiratory symptoms such as headache, sore throat, fever, and cough, but there are also reports of individual deaths. It can happen all year round, but mostly in autumn and winter. A large amount of clinical data have now shown that Mycoplasma pneumoniae infection not only causes respiratory infectious diseases, but also causes many extr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/31
Inventor 胡德明刘清波何林阳辉
Owner SHENZHEN YHLO BIOTECH
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