Aflatoxin B1 degradation agent and application thereof

Abstract

The invention discloses an aflatoxin B1 degradation agent comprising a component a and a component b with a combination ratio of 2 : 3, wherein the component a is prepared by individually culturing lactobacillus casei CGMCC1.62, Bacillus subtilis CGMCC1.504, and Hansenula anomala CGMCC2.881 for 48 hours, mixing with a ratio of 2 : 1 : 1, adding a carrier, and drying under a low temperature; and the component b is prepared by using AFB1 as a carbon source and energy to screen AFB1 degrading bacteria strains, a 26S rDNA identification result showing that a fungi: Aspergillus oryzae is screened to be capable of degrading the AFB1 and the Aspergillus oryzae being with an access No. CGMCC5817 in the China microbiological culture collection center; performing a solid fermentation culture for the AFB1 degrading fungi, extracting a crude enzyme solution of AFB1 degrading enzymes, adding a carrier, and drying under the low temperature. The aflatoxin B1 degradation agent establishes foundations for an application thereof in animal production, pathogenic mechanism researching of AFB1 on a genetic level, and measures taken to mitigate the harm.

Description

technical field [0001] The invention belongs to the technical field of poultry breeding, and in particular relates to an aflatoxin B1 degradation agent and an application thereof. Background technique [0002] The food crops polluted by mycotoxins account for more than 1 / 4 of the total food production in the world every year. It is urgent to take measures to inhibit the production of mycotoxins and detoxify the contaminated food and feed to reduce losses and protect animal and human health. Require. At present, the widely used detoxification method in animal production is to detoxify the contaminated food and feed crops by adding adsorbents. However, this method is mostly the chelation of the adsorbent and the mycotoxin. Although the mycotoxin can be effectively adsorbed, this simple adsorption is very easy to dissociate from the adsorbent when the conditions change and the toxicity still exists. The additive will also adsorb some nutrients in the feed and affect its utili...

AI Technical Summary

Problems solved by technology

However, this method is mostly the chelation of the adsorbent and the mycotoxin. Although the mycotoxin can be effectively adsorbed, this simple adsorption is very easy to dissociate from the adsorbent when the conditions change and the toxicity still exists. The additive will also adsorb some nutrients in the feed and affect its utilization

Images