Chip level PCR-LVCE integrated system

A technology of PCR-LVCE and integrated system, which is applied in the field of electrochemical detection, programmable on-chip system and micro-electromechanical system, and can solve problems such as difficult optimization

Inactive Publication Date: 2013-07-10
HUBEI UNIV FOR NATITIES
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

To realize the detection and control of microfluidic chips, it is usually realized by hard-core processors or solid-core processors, su

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  • Chip level PCR-LVCE integrated system
  • Chip level PCR-LVCE integrated system
  • Chip level PCR-LVCE integrated system

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Embodiment Construction

[0090] The preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings; it should be understood that the preferred embodiments are only for illustrating the present invention, rather than limiting the protection scope of the present invention.

[0091] Fig. 1 shows a schematic structural diagram of a chip-level PCR-LVCE integrated system, and Fig. 2 shows a schematic structural diagram of a chip-level PCR-LVCE microfluidic chip, as can be seen from Fig. 33. The mixing channel 34 and the PCR micro-reaction chamber 36 are connected sequentially. The PCR micro-reaction chamber 36 is also provided with a PCR micro-reaction chamber inlet 35 and an output port 38 after PCR reaction amplification; the PCR micro-heater 37 is arranged in the PCR micro-reaction chamber 36 outside; the waste liquid input port 41 is connected with the waste liquid output port 47 through the micro-separation channel 44, and the micro-separat...

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Abstract

The invention discloses a chip level PCR-LVCE integrated system which comprises a chip level PCR-LVCE micro fluidic chip, a chip level PCR-LVCE detection and a control interface circuit as well as a chip level PCR-LVCE detection and control system based on an SOPC (system on programmable chip) technique; the integrated system achieves ionophortic separation and electrochemical detection of different components of to-be-measured test samples in NIOSII soft core processor control, to-be-analyzed test sample micro mixing of relevant interface circuit control, micro fluid liquid direction judgment in an LVCE chip and variation field intensity forming movements on an LVCE separation micro channel. The chip level PCR-LVCE integrated system provided by the invention has the advantages of rapidity, simplicity, convenience, high integrated degree and the like, can effectively improve the detection efficiency, are higher in expansibility, real-time property and safety compared with the prior art, and can be widely applied to the fields of biochemistry, medicine, environment, criminal investigation and the like.

Description

technical field [0001] The invention relates to the fields of micro-electromechanical systems, electrochemical detection and programmable on-chip systems, and specifically designs a chip-level PCR-LVCE detection and control integrated system. Background technique [0002] Microfluidic chips developed on the basis of micro-fabrication technology of micro-electro-mechanical systems (MEMS) have attracted extensive attention in the fields of life sciences, biochemical detection, and environmental protection because of their advantages of high integration, high efficiency, fast speed, and trace volume. It has become one of the research frontier hotspots in analytical science. Miniaturized Total Analysis Systems (uTAS) is the ultimate development direction of microfluidic chips. uTAS is to realize the integration, automation and portability of biochemical analysis systems from sample processing to detection, that is, sample collection, pre-processing The processing, reaction, sep...

Claims

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Application Information

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IPC IPC(8): C12M1/36C12M1/34
Inventor 廖红华徐建廖宇方芳高林
Owner HUBEI UNIV FOR NATITIES
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