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Method for simultaneous determination of caffeic acid and 8 flavonoids in propolis by using high performance liquid chromatography

A technology of high-performance liquid chromatography and flavonoids, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that have not been reported in the literature, achieve accurate results, simple methods, and benefit the quality control of propolis

Inactive Publication Date: 2013-08-21
杭州蜂之语蜂业股份有限公司
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Problems solved by technology

[0005] At present, there is no literature report on simultaneous quantitative determination of flavonoids and caffeic acid

Method used

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  • Method for simultaneous determination of caffeic acid and 8 flavonoids in propolis by using high performance liquid chromatography
  • Method for simultaneous determination of caffeic acid and 8 flavonoids in propolis by using high performance liquid chromatography
  • Method for simultaneous determination of caffeic acid and 8 flavonoids in propolis by using high performance liquid chromatography

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Embodiment Construction

[0026] The present invention will be further described in detail below in conjunction with the accompanying drawings and examples. The following examples are explanations of the present invention and the present invention is not limited to the following examples.

[0027] Example.

[0028] see Figure 1 to Figure 3 , the method for the simultaneous determination of caffeic acid and 8 kinds of flavonoids in propolis by high performance liquid chromatography in the present embodiment includes a standard stock solution preparation process, a standard intermediate liquid preparation process, a standard use liquid preparation process, a standard curve production process, and a sample Processing procedure and sample measurement procedure.

[0029] The method among the present invention adopts high performance liquid chromatograph to measure, and the high performance liquid chromatograph in the present embodiment is Shimadzu LC-10A high performance liquid chromatograph, and this hig...

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Abstract

The invention relates to a method for simultaneous determination of caffeic acid and 8 flavonoids in propolis by using high performance liquid chromatography. Simultaneous quantitative determination of flavonoids and caffeic acid has not been reported so far. The method provided by the invention is characterized in that a high performance liquid chromatograph is used for determination, the method comprises the procedures consisting of preparation of standard stock solutions, preparation of standard intermediate solutions, preparation of standard working solutions, drafting of standard curves, treatment of samples and determination of the samples, and in the procedure of preparation of the standard stock solutions, a caffeic acid standard stock solution, a rutin standard stock solution, a quercetin standard stock solution, a myricetin standard stock solution, an apigenin standard stock solution, a kaempferol standard stock solution, a pinocembrin standard stock solution, a chrysin standard stock solution and a galangin standard stock solution can be obtained. According to the invention, high performance liquid chromatography is employed for simultaneous determination of caffeic acid and 8 flavonoids in propolis, and pre-treatment of samples is easy and convenient and is rapid to operate.

Description

technical field [0001] The invention relates to a method for simultaneously measuring caffeic acid and 8 kinds of flavonoids in propolis, in particular to a method for simultaneously measuring caffeic acid and 8 kinds of flavonoids in propolis by high performance liquid chromatography, and the 8 kinds of flavonoids are They are rutin, quercetin, myricetin, apigenin, canphenanthrene, pinenin, coyne and galangin. Background technique [0002] Propolis is a viscous substance that bees collect from plant resin and mix with their own secretions. Propolis has the functions of invigorating weakness, reducing turbid fat, and quenching thirst; externally used to detoxify and reduce swelling, astringent and granulation. Propolis has been widely used as a health food raw material. [0003] Flavonoids are recognized as the active components in propolis. The functional components of health food made of propolis are total flavonoids. Propolis also contains phenolic acids. Among them, ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 周萍
Owner 杭州蜂之语蜂业股份有限公司
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