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High-throughput drug and poison rapid detection chip and system

A technology for detecting chips and drugs, which is applied in the field of high-throughput drug and poison rapid detection chips and systems, and can solve problems such as cumbersome operations, complex drug components, and unsatisfactory detection methods for single drug molecules.

Active Publication Date: 2015-09-23
SHANGHAI CRIMINAL SCI TECH RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, gas chromatography-mass spectrometry (GC-MS) detection method is the standard method for drug detection, but it has the following disadvantages: 1. The instrument is expensive, and it cannot be purchased by general units, and it is not easy to popularize; 2. The detection instrument must be used in a good laboratory. Strict training is required for the operation of the instrument; 3. The extraction and derivatization of the inspection materials are required, and the operation is cumbersome and takes a long time; 4. The above-mentioned instruments cannot be quickly used for on-site detection
However, studies have proved that most drugs are small molecules and do not have immunogenicity, so they cannot be used to directly immunize animals to produce antibodies. In addition, the drug composition of drug addicts is complex, and the detection method for a single drug molecule is far from meeting the requirements of current drugs. criminal investigation needs
[0005] Therefore, there is currently no mature, effective and rapid method for detecting multiple drugs in this field, so there is an urgent need to develop a method and system for the rapid detection of multiple drugs at one time.

Method used

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  • High-throughput drug and poison rapid detection chip and system
  • High-throughput drug and poison rapid detection chip and system
  • High-throughput drug and poison rapid detection chip and system

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0053] Preparation of Complete Antigen of Small Molecule Drugs

[0054]The preparation of monoclonal antibodies must be immunized between immunogenic macromolecular proteins to produce monoclonal antibodies. Both drugs and poisons are haptens with small molecular weights and have no immunogenicity themselves, so they cannot be used to directly immunize animals. antibody. In order to obtain monoclonal antibodies against small molecular substances of drug poisons, it is first necessary to prepare complete antigens with immunogenicity. Specifically, the small molecule is activated first, and an active group is introduced into its structure to make it have active chemical properties. In a preferred example of the present invention, it is preferable to introduce an amino group or a carboxyl group on the drug small molecule so as to undergo a condensation reaction with the macromolecular carrier protein to form a complete antigen, and then use a homologous bifunctional amino coupli...

Embodiment 1

[0167] The preparation, purification and identification of embodiment 1 monoclonal antibody

[0168] 1.1 Preparation of immune reagents:

[0169] First emulsify the antigen with Freund's adjuvant, prepare the complete antigen ketamine-KLH with PBS to make a 1mg / mL solution, then mix the complete antigen solution and Freund's adjuvant in equal volumes, and oscillate with a high-speed shaker to form Uniform emulsion, this emulsion is used for animal immunization.

[0170] 1.2 Mice immunization:

[0171] Select 8-week-old mice for injection immunization, take 12 healthy Balb / C mice 8 weeks old, and inject 100ul of emulsified antigen in complete Freund's adjuvant into each mouse intraperitoneally on day 0; The mice were intraperitoneally injected with incomplete Freund's adjuvant emulsified antigen 100ul; on the 21st day, the blood was collected by picking the mouse eyeball, and the antibody titer in the serum was detected by ELISA method; on the 28th day, the incomplete Freund'...

Embodiment 2

[0203] Example 2 Antibody Specificity Determination

[0204] Control samples for cross-reaction experiments were 62 drugs and drugs - methamphetamine, amphetamine, MDMA, MDA, morphine, codeine, heroin, pholcodine, dihydroetorphine, rametidine, fen Tenyl, Tramadol, Dextropropoxyphene, Naloxone, Naltrexone, Nalomorphine, Lofexidine, Scopolamine, Yian Oral Liquid, Zhengtongning Tablets, Paracetamol, Aspirin, Ibuprofen , amitriptyline, imipramine, chlorpromazine, promethazine, diazepam, triazolam, alprazolam, barbiturate, fast sleep, amobarbital, caffeine, norfloxacin, Pioneer IV, berberine, lactose, procaine, Kangfuxin capsules, chloral hydrate, ofofloxacin, phenacetin, pain relief tablets, ketamine, methadone, methadol, meperidine, ephedrine, pseudoephedrine, Cocaine, thebaine, lidocaine, narcotine, buprenorphine, phenylpropanolamine, phenethylamine, oxycodone, hydromorphone, dextropropoxyphene, phencyclidine, methaqualone (methadone). The above 62 kinds of drug and drug-coat...

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Abstract

The invention relates to a high-throughput drug rapid detection chip and a system. In particular, the invention prepares a drug detection chip for the first time. The chip comprises a substrate and a detecting point on the substrate. The detecting point is fixed with a detection antibody specifically bonded with at least four drugs selected from the following group: morphine, amphetamine, methamphetamine, marijuana, ketamine, buprenorphine, methadone, cocaine, MDMA, triazolam, barbital, LSD, fentanyl, a TCA (tricyclic antidepressant), and pethidine. The detection antibody is immunoglobulin induced by a complete antigen molecule. The chip provided in the invention can realize high-throughput simultaneous detection of multiple drugs at one time, has the advantages of high degree of automation, strong specificity and high sensitivity, and can be applied to qualitative and quantitative detection.

Description

technical field [0001] The invention relates to the field of detection of prohibited drugs, in particular to a high-throughput rapid detection chip and system for drug poisons. Background technique [0002] As one of the most serious public hazards in the world today, drug abuse has attracted more and more attention from the international community. According to relevant data from the United Nations, the drug problem has posed a major threat to the survival and development of human beings. At least 51 million people in the world have suffered from drugs, and the annual global drug trade volume has exceeded 500 billion US dollars. In recent years, the drug problem in our country has become increasingly serious, and the trend of drug abuse has become more rapid. The drug crime situation in Shanghai is also very serious. [0003] In the fight against drug crimes, drug testing plays an extremely important role. It not only provides clues for the detection of drug crimes, but a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577
Inventor 曾立波陈连康胡小龙张玉荣
Owner SHANGHAI CRIMINAL SCI TECH RES INST
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