Preparation method of carbon quantum dot test paper strip for detecting P24 antigen

A technology of carbon quantum dots and P24, which is applied in the field of test strips for detecting P24 antigens, can solve the problems of low sensitivity and toxicity, and achieve the effect of high sensitivity

Inactive Publication Date: 2013-10-09
湖南美生医疗健康产业股份有限公司
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Problems solved by technology

This form of test strip not only has low sensitivity due to the colloidal gold pad, but also contains chloroauric acid in the raw material of the colloidal gold, which is toxic

Method used

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  • Preparation method of carbon quantum dot test paper strip for detecting P24 antigen
  • Preparation method of carbon quantum dot test paper strip for detecting P24 antigen
  • Preparation method of carbon quantum dot test paper strip for detecting P24 antigen

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Embodiment

[0057] Collect 50mg of carbon ash and 30ml of 5mol / L nitric acid solution in a three-neck shaker flask and mix them evenly. Reflux the mixed solution for 12h under magnetic stirring at 100°C, then centrifuge at 3000rpm and 4°C for 10min to separate the unreacted carbon After ash, collect the clear brown-yellow supernatant in the solution, discard the precipitate, then add an acetone solution 3 times the volume of the supernatant, centrifuge at 14000rpm for 10 min, discard the supernatant, and collect the precipitate, which is carbon quantum dots; After the precipitate was dried, it was dissolved in deionized water, and the pH was adjusted to 7.0 with sodium carbonate solution. The solution was centrifuged at 4°C and 12,000 rpm for 30 minutes, and the supernatant was collected and dialyzed in a dialysis bag for 48 hours to remove impurities and obtain purified about 2 -6nm water-soluble carbon quantum dot solution, ready for use.

[0058] At the same time, on the one hand, add ...

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Abstract

The invention relates to a test paper strip for detecting a P24 antigen and particularly relates to a preparation method of a carbon quantum dot test paper strip for detecting a P24 antigen. The preparation method comprises the following steps of: bonding a sample pad and a water absorption pad respectively at two ends of a bottom plate, preparing a P24 carbon quantum dot pad and a P24 nitrocellulose membrane, bonding the sample pad, the P24 carbon quantum dot pad, the P24 nitrocellulose membrane and the water absorption pad in a lapping way in sequence on the bottom plate to obtain a test paper plate, cutting the test paper plate into a plurality of test paper strips conforming to defined widths, and hermetically storing the test paper strips. According to the invention, a known specific antibody or antigen is fixed in a zone of the cellulose membrane at first, a sample to be detected is added on the sample pad, a carbon quantum dot marking reagent on the carbon quantum dot pad is dissolved, and a large amount of carbon quantum dots are gathered on a detection band, so that bright light is emitted and specific immunologic diagnosis is achieved. The method is high in sensitivity and low in toxicity or free from toxicity in a detection process.

Description

[0001] technical field [0002] The invention relates to a test strip for detecting the P24 antigen, in particular to a preparation method for the test strip for detecting the P24 antigen. Background technique [0003] After the body is infected with HIV, the P24 antigen is an early pathogenic marker that can be detected in serum, and can be detected 2-3 weeks after infection. At present, test strips are generally used to detect P24 antibodies, and the test strips are mainly bonded with sample pads, colloidal gold pads, coating films and absorbent pads on the bottom plate in sequence. The processing of the sample pad is to put the glass fiber membrane into the sample pad treatment solution containing BSA and PBS; the preparation of the coating film is to dilute the antigen with the coating buffer solution and then print on the coating film; the preparation of the colloidal gold pad is to use Potassium carbonate adjusts the colloidal gold solution, and recombines the solutio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
Inventor 胡敏潘艺梁好
Owner 湖南美生医疗健康产业股份有限公司
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