A kind of preparation method of carbon quantum dot test strip for detecting p24 antigen

Abstract

The invention relates to a test paper strip for detecting a P24 antigen and particularly relates to a preparation method of a carbon quantum dot test paper strip for detecting a P24 antigen. The preparation method comprises the following steps of: bonding a sample pad and a water absorption pad respectively at two ends of a bottom plate, preparing a P24 carbon quantum dot pad and a P24 nitrocellulose membrane, bonding the sample pad, the P24 carbon quantum dot pad, the P24 nitrocellulose membrane and the water absorption pad in a lapping way in sequence on the bottom plate to obtain a test paper plate, cutting the test paper plate into a plurality of test paper strips conforming to defined widths, and hermetically storing the test paper strips. According to the invention, a known specific antibody or antigen is fixed in a zone of the cellulose membrane at first, a sample to be detected is added on the sample pad, a carbon quantum dot marking reagent on the carbon quantum dot pad is dissolved, and a large amount of carbon quantum dots are gathered on a detection band, so that bright light is emitted and specific immunologic diagnosis is achieved. The method is high in sensitivity and low in toxicity or free from toxicity in a detection process.

Description

technical field

[0001] The invention relates to a test strip for detecting the P24 antigen, in particular to a preparation method for the test strip for detecting the P24 antigen. Background technique

[0002] After the body is infected with HIV, the P24 antigen is an early pathogenic marker that can be detected in serum, and can be detected 2-3 weeks after infection. At present, test strips are generally used to detect P24 antibodies, and the test strips are mainly bonded with sample pads, colloidal gold pads, coating films and absorbent pads on the bottom plate in sequence. The processing of the sample pad is to put the glass fiber membrane into the sample pad treatment solution containing BSA and PBS; the preparation of the coating film is to dilute the antigen with the coating buffer solution and then print on the coating film; the preparation of the colloidal gold pad is to use Potassium carbonate adjusts the colloidal gold solution, and recombines the solution with ot...

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