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Microscope temperature-controlled slide and method for observing morphological changes of antifreeze protein ice crystals using optical microscope

An antifreeze protein and microscope technology, which is applied to the observation of ice crystal morphological changes in an ordinary optical microscope and the observation field of ice crystal morphological changes in antifreeze protein solutions, can solve problems such as high requirements for instruments and equipment, and achieve convenient observation, good temperature control effect, Easy to use effects

Inactive Publication Date: 2015-08-26
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the improved method also has its shortcomings. It has high requirements for equipment. In addition to using a phase contrast microscope, a special stainless steel sample holder is required.

Method used

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  • Microscope temperature-controlled slide and method for observing morphological changes of antifreeze protein ice crystals using optical microscope
  • Microscope temperature-controlled slide and method for observing morphological changes of antifreeze protein ice crystals using optical microscope
  • Microscope temperature-controlled slide and method for observing morphological changes of antifreeze protein ice crystals using optical microscope

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: See figure 1 , A temperature-controlled glass slide for a microscope, a cooling hose 3 is arranged between two slides 1 and the two ends of the cooling hose 3 are respectively connected to the outlet 4-1 and the inlet 4- of the cryogenic cooling cycler 4 2 phase connection, two slides are fixed by two clamps 2 can also be fixed and adjusted with bolts. The low-temperature cooling cycler is the DHX-2020 model of Van Dilang brand, with a temperature range of -20-100°C and an accuracy of ±0.05°C.

[0033] The low-temperature cooling cycler 4 includes a loaded refrigerant, an evaporator, and a compressor, and a circulating pump is provided between the outlet and the evaporator.

Embodiment 2

[0034] Example 2: See figure 2 , A temperature-controlled glass slide for a microscope, a cooling hose 3 is arranged between two slides 1, and the outlet 3-1 of the cooling hose 3 is connected with the inlet end of the peristaltic pump 5, and the outlet end of the peristaltic pump 5 Connect the inlet end of the insulation box 6, and the outlet end of the insulation box 6 is connected with the inlet 3-2 of the cooling hose 3; a rapid detection probe thermometer 7 is installed on the slide 1; in the insulation box 6, there is -20℃ ~-18℃ absolute ethanol 8, the inlet tube 6-2 and outlet tube 6-1 of the insulation box 6 are respectively inserted into the -20℃~-18℃ absolute ethanol 8, two slides are fixed by two clips You can also use bolts to fix and adjust. Longer / Lange BT100-2J peristaltic pump, display speed, speed range: 0.1-100rpm, flow range: 0.007-380ml / min.

[0035] To use a peristaltic pump, a quick detection probe thermometer is required, the temperature range is -40℃~150...

Embodiment 3

[0036] Example 3: A method for observing the morphological changes of antifreeze protein ice crystals using an ordinary optical microscope, including the following steps:

[0037] (1) Extraction of antifreeze protein; according to conventional or standard extraction.

[0038] (2). Prepare the temperature-controlled glass slides of the microscope; connect the cooling hose 2 between the two glass slides 1, and connect the outlet 3-1 of the cooling hose 3 to the inlet end of the peristaltic pump 5. The outlet end is connected to the inlet end of the insulation box 6, and the outlet end of the insulation box 6 is connected to the inlet 3-2 of the cooling hose 3; a rapid detection probe thermometer 7 is provided on the slide glass 1; in the insulation box 6 there is- Anhydrous ethanol 8 at 20°C to -18°C, the inlet pipe 6-1 and outlet pipe 6-2 of the heat preservation box 6 are respectively inserted into anhydrous ethanol 8 at -20°C to -18°C. Longer / Lange BT100-2J peristaltic pump, disp...

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Abstract

The invention relates to the technical field of observation of ice crystal morphologies, and particularly relates to a method for observing the ice crystal morphology change by using a common optical microscope. A temperature-controlled slide of a microscope is mainly characterized in that a cooling hose is arranged between two glass slides; two ends of the cooling hose are respectively connected with an outlet and an inlet of a low-temperature cooling circulator. The method for observing the ice crystal morphology change of the antifreeze protein by using the common optical microscope is mainly characterized by comprising the following steps: (1) extracting the antifreeze protein; (2) preparing the temperature-controlled slide of the microscope; (3) observing the ice crystal morphology change, and observing the ice crystal growth process.

Description

Technical field [0001] The invention relates to the technical field of ice crystal morphology observation, in particular to the observation of ice crystal morphology changes in an antifreeze protein solution, and a method for observing ice crystal morphology changes by using an ordinary optical microscope. Background technique [0002] Low temperature stress includes cold stress between 0℃-12℃ and freezing stress below 0℃. Changes in environmental temperature will cause physical and chemical changes of substances in aqueous solutions. Cell icing caused by low temperature is often accompanied by dehydration. The osmotic pressure inside and outside the cell increases, the intracellular concentration increases, the cell volume shrinks, the plasma membrane system and cytoskeleton are damaged, the activity of biological macromolecules changes, and the function is lost, which is harmful. Material accumulation affects the structure and function of plant organelles such as mitochondria, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/01
Inventor 孙万仓杨宁宁史鹏辉孔德晶刘自刚方彦曾秀存武军艳鲁美宏杨刚王月王丽萍赵艳宁董红业
Owner GANSU AGRI UNIV
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