Cellar mechanical simulation system for researching tumor cell epithelium-mesenchyma conversion

Abstract

The invention discloses a cellar mechanical simulation system for researching tumor cell epithelium-mesenchyma conversion. The cellar mechanical simulation system for researching tumor cell epithelium-mesenchyma conversion comprises a constant flow pump, a liquid storage bottle and an in-vitro cellar mechanical stimulation device, and the constant flow pump, the liquid storage bottle and the in-vitro cellar mechanical stimulation device are connected in series through a conduit to form a closed ring. Compared with the prior art, the cellar mechanical simulation system for researching tumor cell epithelium-mesenchyma conversion allows a plurality of glass slides to be disposed, tumor cells planted on the glass slides to undergo shear stress loading, the loaded flow field to be uniform and stable and the shear stress loading having different sizes to be realized through the flow adjustment. The system can be used for observing the dynamic situation of the epithelium-mesenchyma conversion of the tumor cells planted on the glass slides after the action of the fluid shear stress and observing the local migration and adherence capability of the shear stress acted upstream cells to the downstream through the series-connected glass slides.

Description

technical field [0001] The invention relates to an industrialized simulation system, in particular to a cell mechanics simulation system for studying tumor cell epithelial-mesenchymal transition. Background technique [0002] Malignant tumors are tumors in which cells not only proliferate abnormally and rapidly, but also spread and metastasize. Most of the deaths of clinical cancer patients are caused by tumor metastasis, so we need to study the general process and law of tumor metastasis. [0003] Currently, models related to tumor metastasis include two categories: in vitro models and in vivo models. In vitro models are mostly invasion or adhesion models, while in vivo models are mainly metastasis models, including artificial transfer and spontaneous transfer models. At present, most of the hosts of in vivo models are mice, and due to the emergence of nude mice, it is more convenient to study human tumors in mice. However, the in vivo model also has obvious limitations....

AI Technical Summary

Problems solved by technology

Third, most of the current flow chambers are only designed for a single slide or a small number of cells. If the amount of cells required is large, the repeatability of the experiment is high, or because the experiment requires a large shear stress area such as If multiple slides are connected in series, the current flow chamber cannot meet the requirements

If the flow chamber is used in a cycle or in series, it is time-consuming and laborious, and may also change the experimental environment, and also add more uncertain factors, which will affect the results

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