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Tissue culture rapid propagation method for wild sweet cherry trees

A technology for tissue culture fast propagation and sweet cherries, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as inability to meet development and production, inability to maintain excellent traits of the female parent, degradation, etc., and reduce pests and diseases. The effect of occurrence, low cost and high work efficiency

Active Publication Date: 2013-12-04
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of local wild sweet cherry is mostly wild in the mountains, without development, and the propagation method is mostly natural seed propagation or cutting. Good traits

Method used

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  • Tissue culture rapid propagation method for wild sweet cherry trees
  • Tissue culture rapid propagation method for wild sweet cherry trees
  • Tissue culture rapid propagation method for wild sweet cherry trees

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: a kind of method of wild sweet cherry wood tissue culture rapid propagation, it may further comprise the steps:

[0019] S1. Cut the explants: Collect the year-old branches of wild sweet cherries without disease at noon or afternoon on a sunny day, remove excess leaves, cut out the stems with buds, rinse them in running water for 5 minutes, and dry the water;

[0020] S2. Sterilization of explants: put the washed explants into the ultra-clean workbench, disinfect them with 70% alcohol for 25 seconds, rinse them with sterile water for 4 times, and then put them in mercuric chloride with a concentration of 0.1% Sterilize in medium for 3 minutes, rinse 4 times with sterile water, and dry on sterile paper;

[0021] S3. Induction culture: Inoculate the sterilized explants in the induction medium for cultivation, wherein the induction medium is MS+6-BA3.0mg / L+IAA0.5mg / L+sucrose 30g / L+agar 10g / L L, the pH value is 5.5, the culture conditions are: light intensity...

Embodiment 2

[0025] Embodiment 2: a kind of method for the rapid propagation of wild sweet cherry wood tissue culture, it may further comprise the steps:

[0026] S1. Cut the explants: collect the year-old branches of wild sweet cherries without disease at noon or afternoon on a sunny day, remove excess leaves, cut out the stems with buds, rinse them in running water for 10 minutes, and then absorb the water;

[0027] S2. Sterilization of explants: put the washed explants into the ultra-clean workbench, disinfect with 75% alcohol for 35 seconds, rinse with sterile water for 6 times, and then put mercuric chloride with a concentration of 0.2% Sterilize in medium for 8 minutes, rinse 6 times with sterile water, and dry on sterile paper;

[0028] S3. Induction culture: Inoculate the sterilized explants in the induction medium for cultivation, wherein the induction medium is MS+6-BA3.0mg / L+IAA0.5mg / L+sucrose 30g / L+agar 10g / L L, the pH value is 6.5, and the culture conditions are: light intens...

Embodiment 3

[0031] Embodiment 3: a kind of method of wild sweet cherry wood tissue culture rapid propagation, it may further comprise the steps:

[0032] S1. Cut out the explants: collect the year-old branches of wild sweet cherries without disease at noon or afternoon on a sunny day, remove excess leaves, cut out the stems with buds, rinse them in running water for 8 minutes, and then absorb the water;

[0033] S2. Sterilization of explants: put the washed explants into the ultra-clean workbench, disinfect them with 73% alcohol for 30 seconds, rinse them with sterile water for 5 times, and then put them in mercuric chloride with a concentration of 0.15% Sterilize in medium for 5 minutes, rinse 5 times with sterile water, and dry on sterile paper;

[0034]S3. Induction culture: Inoculate the sterilized explants in the induction medium for cultivation, wherein the induction medium is MS+6-BA3.0mg / L+IAA0.5mg / L+sucrose 30g / L+agar 10g / L L, the pH value is 6, the culture conditions are: light...

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Abstract

The invention discloses a tissue culture rapid propagation method for wild sweet cherry trees. The method comprises the following steps: S1, explant cutting; S2, explant sterilization; S3, induction culture, that is a sterilized stem with buds is inoculated in an induction culture medium for cultivation, and then an axillary bud can be induced to sprout; S4, successive transfer culture, that is a sprouting axillary bud is inoculated in a successive transfer culture medium for cultivation, and then a cluster bud can be induced to generate; S5, rooting culture, that is the cluster bud which is cut is inoculated in a rooting culture medium for cultivation so as to be induced to root; S6, hardening-seedling transplantation. According to the invention, a sterile seedling of local wild sweet cherry in Nanjiang County, Bazhong City of Sichuan Province can be obtained successfully through the way of tissue culture propagation, a tissue culture rapid propagation is established, a great amount of sterile offsprings can be obtained in a short time, and not only can the indigenous excellent properties of a female parent be kept, but also the plant diseases and insect pests can be reduced, so that base material is provided for realizing large-scale industrialized production of wild sweet cherry.

Description

technical field [0001] The invention relates to a method for cultivating plants, in particular to a method for tissue culture and rapid propagation of wild sweet cherry wood. Background technique [0002] Plant tissue culture refers to the cultivation of various parts of plants, such as cambium, parenchyma, mesophyll tissue, endosperm, etc. to obtain regenerated plants, and also refers to the cultivation of callus produced from various organs during the culture process, and the callus regenerated Regenerated plants are formed after redifferentiation. The plant tissue culture method can quickly reproduce a variety of plants in a short period of time. Not only the reproduction rate is extremely high, but also because it is asexual reproduction, it can maintain the excellent shape of the original propagating wood. In recent years, it has been more and more widely used in production. The principle of plant tissue culture technology is to use the totipotency of plant cells, that...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 马建华王小辉朱晓菲沈香兰吴佳川
Owner SICHUAN COLORLINK CO LTD
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