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Culture vessel for forming embryoid body

A technology of culture container and embryo body, which is applied in tissue cell/virus culture devices, bioreactors/fermenters for specific purposes, biochemical instruments, etc., can solve the problems of cumbersome operation, small EB volume, and low success rate.

Inactive Publication Date: 2013-12-11
SUMITOMO BAKELITE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the pendant drop method, there are problems such as low success rate of EB body formation, inability to observe with a microscope, small amount of EB bodies that can be formed at one time, and cumbersome operation.

Method used

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  • Culture vessel for forming embryoid body
  • Culture vessel for forming embryoid body
  • Culture vessel for forming embryoid body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～5）

[0088] Using polystyrene resin (manufactured by PS Japan Co., Ltd., HF77) resin as a resin material, a 96-well multiwell plate was molded by injection molding. The shape of each hole is as figure 1 As shown, in Examples 1 to 5, the angles of the diameter-reduced portions were set to 3°, 5°, 10°, 20°, and 30°, respectively.

[0089] The obtained plate was subjected to plasma treatment (oxygen plasma for 10 minutes) using a plasma treatment apparatus (SERIES 7000 manufactured by BRANSON / IPC), and wettability was imparted to the surface of the plate as a pretreatment.

[0090] Next, as a water-soluble resin, polyvinyl alcohol (manufactured by Toyo Gosei Kogyo Co., Ltd. AWP: a compound of formula (Ia)) having an azide group in the side chain (the average degree of polymerization of the water-soluble resin is 1600, the introduction rate of the photosensitive group 0.65mol%)), dissolved in a 25vol% aqueous ethanol solution in a polypropylene container shaded with a colored resin to...

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Abstract

The present invention provides a culture vessel for forming an embryoid body, which can form a high-quality embryoid body at a higher level and with higher efficiency. The present invention relates to a culture vessel for forming an embryoid body, which has at least two wells and is characterized in that each of the wells has a bottom having an approximately U-shaped cross section when observed in a vertical direction and an opening having an approximately circular form, at least the curved part of the inner surface of the bottom has poorly cell-adhesive properties, the curvature radius (R') of the inner surface of the bottom is 1.0 to 3.5 mm inclusive, the surface having poorly cell-adhesive properties is formed by a treatment for making a surface poorly cell-adhesive or a treatment for making a surface non-cell-adhesive each using a water-soluble resin.

Description

technical field [0001] The present invention relates to a culture container for embryoid body formation. [0002] This application claims priority based on Japanese Patent Application No. 2011-76621 for which it applied in Japan on March 30, 2011, and uses the content here. Background technique [0003] Embryonic stem cells (embryonic stem cells: ES cells) are obtained by inoculating the inner cell mass of blastocysts on feeder cells and adding leukemia inhibitory factor (leukemia inhibitory factor: LIF) for culture. [0004] ES cells that maintain an undifferentiated state and proliferate indefinitely have the pluripotency to differentiate into various tissue cells, so they are the subject of various researches in the fields of transplantation therapy using differentiated tissues and so-called regenerative medicine . [0005] In order to induce differentiation of ES cells into various tissues, the most widely used method is to form a pseudo-embryo called an embryoid body ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
CPCC12M21/06C12M23/12C12M23/20C12M3/00C12N5/0603
Inventor 佐仓武司塚田亮平
Owner SUMITOMO BAKELITE CO LTD