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Material including royal jelly-decomposing enzyme

A technology of royal jelly and decomposed products, applied in hydrolytic enzymes, medical preparations containing active ingredients, food science, etc., can solve undiscovered problems and achieve excellent functional and digestibility effects

Inactive Publication Date: 2013-12-25
AKITAYAHONTEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned problems can be solved by using protease etc. using royal jelly as a substrate, but such an enzyme has not yet been found

Method used

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Examples

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Embodiment

[0079] Examples are given below to describe the present invention more specifically, but the present invention is not limited to the following examples.

reference example 1

[0080] [Reference Example 1] Preparation of larval suspension

[0081] After collecting about 15 g of 3-day-old queen larvae of Apis mellifera, they were washed with ice-cooled physiological saline. Then, the queen bee larvae were filtrated by using a cloth made of polyester with an average of 100 meshes ("Tetron", manufactured by Toray Co., Ltd.), the cuticles of the larvae were broken, and body fluids and viscera were squeezed out. A milky white larval suspension is thus prepared.

reference example 2

[0082] [Reference Example 2] Preparation of RJ protein solution

[0083] To 10 ml of RJ (produced in China: Zhejiang Jiangshan Hengliang Bee Products Co., Ltd.), 20 mL of 50 mM phosphate buffer at pH 7.0 was added and mixed to prepare a diluted RJ solution. The above diluted RJ solution was centrifuged at 25,000×g at 5° C. for 20 minutes, then insoluble components were removed, and the supernatant was collected. Then, the above supernatant was diluted with 50 mM phosphate buffer at pH 7.0 to prepare a 30 mg / mL RJ protein solution.

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Abstract

The invention relates to a material including royal jelly-decomposing enzyme, a royal jelly-decomposing enzyme, a royal jelly-decomposing material and a decomposing method of royal jelly. This material containing royal jelly-decomposing enzyme is prepared by a method including the following processes from the 2-3 day old queen bee larvae of Apis mellifera: (a) a process of centrifugal treatment of the suspension of body tissues of the queen bee larvae at <=6[deg.]C so as to separate to an upper layer of white solid form, a middle layer of solution and a lower layer of precipitate; and (b) a process of recovering the middle layer as the material containing royal jelly-decomposing enzyme. The royal jerry-decomposing enzyme is characterized by being contained in the above material containing royal jerry-decomposing enzyme. The royal jelly-decomposed products are prepared by using the material containing royal jelly-decomposing enzyme or the royal jelly-decomposing enzyme. The method for decomposing the royal jelly is characterized by using the material containing royal jelly-decomposing enzyme or the royal jelly-decomposing enzyme.

Description

[0001] This application is a divisional application for an invention patent application with an application date of February 22, 2008, an application number of 200810007986.X, and an invention title of "Containing Royal Jelly Decomposing Enzyme". technical field [0002] The present invention relates to a royal jelly-decomposing enzyme-containing material prepared from 2 to 3-day-old queen larvae of Apis mellifera, a royal jelly-decomposing enzyme contained in the above-mentioned royal jelly-decomposing enzyme-containing material, and a method for using the above-mentioned royal jelly-decomposing enzyme-containing material or the above-mentioned A royal jelly decomposed product characterized by preparation of a royal jelly decomposing enzyme, and a method of decomposing royal jelly using the above royal jelly decomposing enzyme-containing material or the above royal jelly decomposing enzyme. Background technique [0003] Royal jelly is a milky white jelly-like nutrient secret...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61K38/46A61P39/06A61P31/00A61P25/00A23L1/29A23L1/305A23L2/66A23L2/38C12N9/14A23L21/20A23L33/00A23L33/10A23L33/115A61K35/644A61P25/28A61P31/04A61P31/12A61P43/00C07K14/435C12N9/50C12P21/00
Inventor 松冈拓磨渡边铃代川岛拓司中村正金丸义敬稻垣瑞穗林要喜知铃木寿三嶋智之
Owner AKITAYAHONTEN
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