Queen gel clastic enzyme composition

A technology of propolis and decomposing enzymes, applied in the directions of hydrolase, medical preparations containing active ingredients, food science, etc., can solve the problems that have not yet been discovered, and achieve the effect of excellent function and digestion and absorption

Inactive Publication Date: 2008-08-27
AKITAYAHONTEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned problems can be solved by using protease etc. using royal jelly as a substrate, but such an enzyme has not yet been found

Method used

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  • Queen gel clastic enzyme composition
  • Queen gel clastic enzyme composition
  • Queen gel clastic enzyme composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0078] Examples are given below to describe the present invention more specifically, but the present invention is not limited to the following examples.

reference example 1

[0079] [Reference Example 1] Preparation of larval suspension

[0080] After collecting about 15 g of 3-day-old queen larvae of Apis mellifera, they were washed with ice-cooled physiological saline. Then, the queen bee larvae were filtered using polyester cloth with an average of 100 meshes ("Tetron", manufactured by Toray Co., Ltd.), the cuticles of the larvae were broken, and the body fluids and viscera were squeezed out. A milky white larval suspension is thus prepared.

reference example 2

[0081] [Reference Example 2] Preparation of RJ protein solution

[0082] 20 mL of 50 mM phosphate buffer at pH 7.0 was added to 10 mL of RJ (produced in China: Zhejiang Jiangshan Hengliang Bee Products Co., Ltd.), and mixed to prepare a diluted RJ solution. The above diluted RJ solution was centrifuged at 25,000×g at 5° C. for 20 minutes, then insoluble components were removed, and the supernatant was collected. Then, the above supernatant was diluted with 50 mM phosphate buffer at pH 7.0 to prepare a 30 mg / mL RJ protein solution.

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Abstract

The invention relates to a queen bee gel decomposition enzyme contained object, a queen bee gel decomposition object and a decomposition method of the queen bee gel. The queen bee gel decomposition enzyme contained object is prepared by grubs of the queen bee living for 2 to 3 days of Apis mellifera via the method comprising the following procedures: (a) performing centrifugal processing to body tissue suspended solid of the grubs of the queen bee below 6 DEG C, to separate into three layers which are white solid upper layer, solution middle layer and deposition lower layer; and (b) recycling the middle layer as the queen bee gel decomposition enzyme contained object. The queen bee gel decomposition enzyme is characterized in that the queen bee gel decomposition enzyme is contained in the queen bee gel decomposition enzyme contained object. The queen bee gel decomposition object is prepared by using the queen bee gel decomposition enzyme contained object or the queen bee gel decomposition enzyme. The decomposition method of the queen bee gel is characterized in that the method uses the queen bee gel decomposition enzyme contained object or the queen bee gel decomposition enzyme.

Description

technical field [0001] The present invention relates to a propolis-decomposing enzyme-containing substance prepared from 2-3-day-old queen bee larvae of Apis mellifera, a propolis-degrading enzyme contained in the above-mentioned propolis-degrading enzyme-containing substance, and the use of the above-mentioned propolis-degrading enzyme A propolis-degrading product characterized in that the product containing the propolis-degrading enzyme is prepared, and a method for decomposing propolis using the product containing the propolis-degrading enzyme or the propolis-degrading enzyme. Background technique [0002] Royal propolis is a milky, gelatinous nutrient secreted by worker bees from the hypopharyngeal and mandible glands for the growth of queen bee larvae. Because queen bees and worker bees are genetically homogeneous, it is believed that royal glue contains some control factors that induce differentiation to queen bees, but the above substances have not been clarified. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/14A61K35/64A61P39/06A61P31/14A61P25/00A23L2/38A23L21/20A23L33/00A23L33/10A23L33/115A61K35/644A61P25/28A61P31/04A61P31/12A61P43/00C07K14/435C12N9/50C12P21/00
Inventor 松冈拓磨渡边铃代川岛拓司中村正金丸义敬稻垣瑞穗林要喜知铃木寿三嶋智之
Owner AKITAYAHONTEN
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