Queen gel clastic enzyme composition
A technology of propolis and decomposing enzymes, applied in the directions of hydrolase, medical preparations containing active ingredients, food science, etc., can solve the problems that have not yet been discovered, and achieve the effect of excellent function and digestion and absorption
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[0078] Examples are given below to describe the present invention more specifically, but the present invention is not limited to the following examples.
reference example 1
[0079] [Reference Example 1] Preparation of larval suspension
[0080] After collecting about 15 g of 3-day-old queen larvae of Apis mellifera, they were washed with ice-cooled physiological saline. Then, the queen bee larvae were filtered using polyester cloth with an average of 100 meshes ("Tetron", manufactured by Toray Co., Ltd.), the cuticles of the larvae were broken, and the body fluids and viscera were squeezed out. A milky white larval suspension is thus prepared.
reference example 2
[0081] [Reference Example 2] Preparation of RJ protein solution
[0082] 20 mL of 50 mM phosphate buffer at pH 7.0 was added to 10 mL of RJ (produced in China: Zhejiang Jiangshan Hengliang Bee Products Co., Ltd.), and mixed to prepare a diluted RJ solution. The above diluted RJ solution was centrifuged at 25,000×g at 5° C. for 20 minutes, then insoluble components were removed, and the supernatant was collected. Then, the above supernatant was diluted with 50 mM phosphate buffer at pH 7.0 to prepare a 30 mg / mL RJ protein solution.
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