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Binding molecules that neutralize rabies virus

A rabies virus and binding molecule technology, applied in the field of binding molecules that can neutralize rabies virus, can solve problems such as short serum half-life and direct drug delivery restrictions

Active Publication Date: 2015-11-25
CELLTRION INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a rabies-virus neutralizing mouse monoclonal antibody has been developed (SchumacherCL et al., J.Clin.Invest.Vol.84, p.971-975, 1989), due to its short serum half-life, it is used to induce human The lack of effector function and the ability to induce unwanted HAMA (humananti-mouseantibody, human anti-mouse antibody) responses to mouse antibodies in humans limits direct administration to human patients infected with rabies

Method used

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  • Binding molecules that neutralize rabies virus
  • Binding molecules that neutralize rabies virus
  • Binding molecules that neutralize rabies virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Embodiment 1: hybridoma (hybridoma) cell screening

[0088] According to the previous experiments and records on hybridoma cells kept in the Center for Disease Control (Center for Disease Control: hereinafter referred to as "CDC"), after screening more than 10 kinds of hybridoma cells, they passed RFFIT (Rapid Fluorescent Focus Inhibition Test, Rapid Fluorescent Focus Inhibition Test) (Smith, J.S. et al., Geneva: World Health Organization, pp.181-191,1996; and Centers for Disease Control and Prevention, Morb.Mortal.WeeklyRep.49(RR-1), 1-21,1999) method to determine the The antigen-binding fragment (Fab) titer (titer) of the culture medium is described in Table 1. As a result, clones above a certain level (0.7IU / ml) were screened, and the names of the clones were as follows: #62-62-5, #62-62-6, #2-21-8, #2-21-14 and # 2-21-23.

[0089] Table 1

[0090] [Table 1]

[0091] Clonal titer test results

[0092] Sample ID

Isotype

Titer

62-111-1

...

Embodiment 2

[0114] Example 2: cDNA determination of chimeric antibodies secreted by hybridoma cells

Embodiment 2-1

[0115] Example 2-1: Cell culture

[0116] Hybridoma cells #2-21-8, 2-21-14, 2-21-23, and 62-80-6 were stored from the CDC in the United States, and the above cells were added with 5% fetal bovine serum (FBS; Sigma, 12003C) in IMDM medium (Invitrogen 12440-053). During the culture period, the Mycoplasma PCR ELISA kit (Mycoplasma PCRELISAkit) (Roche, 11663925910) was used to check whether there was mycoplasma contamination, and it was confirmed that there was no Mycoplasma in the culture medium.

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Abstract

The present invention relates to a binding molecule capable of neutralizing rabies virus. More specifically, the binding molecules of the present invention have the ability to neutralize rabies virus derived from individuals such as dogs, cows, mongooses, bats, skunks and wolves, and thus can be effectively used to treat infections from a wide range of animals. patients with rabies virus.

Description

technical field [0001] The present invention relates to a binding molecule capable of neutralizing rabies virus. Background technique [0002] Rabies (rabies) is a viral zoonotic infectious disease. In addition to mainly affecting wild and pet animals, it also affects mammals including humans and becomes the cause of acute brain diseases. Rabies is a fatal disease that almost leads to death once it occurs, and is considered to be the disease with the highest fatality rate together with AIDS. The above-mentioned rabies is spread all over the world, more than ten million people are infected and treated every year, and the annual death toll is 40,000 to 70,000. [0003] Rabies is transmitted through saliva and blood, mainly when bitten by a rabies-infected dog or cat. Moreover, most mammals such as skunks and bats may also be infected with rabies. [0004] After the rabies virus (rabies virus) reaches the brain nerve tissue through the nerve tissue of the body, it shows the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/42C12N15/13A61K39/395A61P31/14
CPCC07K16/10A61P31/14C07K16/42C12N15/11A61K39/395
Inventor 张宸在金昄谦洪慧真金星贤
Owner CELLTRION INC