Method for determining polynucleotide base sequence and device for determining polynucleotide base sequence

A base sequence and polynucleotide technology, which is applied in the field of determining the base sequence of polynucleotides and devices for determining the base sequence of polynucleotides, and can solve problems such as limited aperture selection and system instability.

Active Publication Date: 2014-01-01
OSAKA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this technology has many problems such as (1) limited choice of ape

Method used

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  • Method for determining polynucleotide base sequence and device for determining polynucleotide base sequence
  • Method for determining polynucleotide base sequence and device for determining polynucleotide base sequence
  • Method for determining polynucleotide base sequence and device for determining polynucleotide base sequence

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Embodiment

[0265]

[0266] Formed using nanofabrication mechanically controlled junction breaking (MCBJ) figure 1 The electrode pair shown (cf. Tsutsui, M., Shoji, K., Taniguchi, M. Kawai, T., Formation and self-breaking mechanism of stable atom-sized junctions. Nano Lett. 8, 345-349 (2007)). Hereinafter, a method for producing the electrode pair will be briefly described.

[0267] Using an electron beam lithography apparatus (manufactured by JEOL Ltd., catalog number: JSM6500F), standard electron beam lithography and lift-off techniques were performed on polyimide (manufactured by Industrial Summit Technology, catalog number: Pyre-Ml) package. Nanoscale gold joints are patterned on a covered flexible metal substrate (phosphor bronze substrate).

[0268] Next, the polyimide located below the joint was removed by etching by a reactive ion etching method using a reactive ion etching apparatus (manufactured by Sumko Corporation, catalog number: 10NR). Then, the substrate was bent to fab...

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Abstract

In the present invention, for each of a plurality of pulses of tunneling current arising when a polynucleotide passes between an electrode pair, the greatest current value and the pulse continuation time are measured, and on the basis of the greatest current value and pulse continuation time, the base sequence of the polynucleotide is determined.

Description

technical field [0001] The present invention relates to a method for determining the base sequence of a polynucleotide and an apparatus for determining the base sequence of a polynucleotide. More specifically, the present invention relates to a method of determining the base sequence of a polynucleotide based on a tunneling current generated when the polynucleotide passes between a pair of electrodes and an apparatus for carrying out the method. Background technique [0002] The technique of analyzing the base sequence of polynucleotides is not limited to the field of academic research, but is also applied to fields such as medical treatment, drug development, and criminal investigation, and interest in the development of this technique is increasing. [0003] Existing sequencers for polynucleotides (specifically, DNA) are based on light measurement technology that recognizes fluorescent labels, and cannot directly recognize the nucleotides themselves that form the polynucle...

Claims

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Application Information

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IPC IPC(8): G01N27/00C12N15/00G16B30/00
CPCC12N15/00G01N33/48721C12Q1/6869G16B30/00C12Q2563/116C12Q2565/607C12Q2565/601
Inventor 川合知二大城敬人松原一喜古桥匡幸筒井真楠谷口正辉
Owner OSAKA UNIV
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