A method for extracting tyrosinase inhibitor from tea peel
The technology of tyrosinase and tea peel is applied in the field of extraction of tyrosinase inhibitors, and achieves the effects of significant inhibition effect, improved extraction efficiency and improved economic benefit.
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Embodiment 1
[0027] Step A: Weigh 20 g of the dried and pulverized tea peel, take the tea peel and the ethanol water solution with a volume concentration of 95% as 1g:20ml, add the ethanol water solution with a volume concentration of 95% to the tea peel, and set the temperature at 50°C After 4 hours of extraction, centrifuge filtration to obtain filter residue and extract, wherein the conditions of centrifugal filtration are 5500rpm, and the centrifugation time is 35min;
[0028] Step B: Take the filter residue obtained in step A and disodium hydrogen phosphate-citric acid buffer as 1g:20ml, add disodium hydrogen phosphate-citric acid buffer to the filter residue obtained in step A, soak at 40°C Extracted for 3 hours, then ultrasonically extracted at a frequency of 200W for 20min, then centrifugally filtered at 3000rpm for 35min to obtain an extract; Sodium-citrate buffer solution, and the activity of cellulase is not less than 25000U / g;
[0029] Step C: Concentrate the extract obtained ...
Embodiment 2
[0032] Step A: Weigh 20 g of the dried and pulverized tea peel, take the tea peel and the ethanol water solution with a volume concentration of 95% as 1g:25ml, add the ethanol water solution with a volume concentration of 95% to the tea peel, and heat at 55°C After extracting for 3.5 hours, centrifuge to obtain filter residue and extract, wherein the condition of centrifuge is 6000rpm, centrifugation time is 30min;
[0033] Step B: Take the filter residue obtained in step A and disodium hydrogen phosphate-citric acid buffer solution as 1g:25ml, add disodium hydrogen phosphate-citric acid buffer solution to the filter residue obtained in step A, soak at 50°C Extracted for 2.5 hours, then ultrasonically extracted at a frequency of 250W for 15min, then centrifuged at 3500rpm for 30min to obtain an extract; the pH of the disodium hydrogen phosphate-citric acid buffer was 4.5, containing 3% Cellulase buffer solution, and the activity of cellulase is not less than 25000U / g;
[0034...
Embodiment 3
[0037] Step A: Weigh 20 g of the dried and pulverized tea peel, take the tea peel and the ethanol water solution with a volume concentration of 95% as 1g:30ml, add the ethanol water solution with a volume concentration of 95% to the tea peel, and heat it at 60°C After 3 hours of extraction, centrifuge filtration to obtain filter residue and extract, wherein the condition of centrifugal filtration is 6500rpm, and the centrifugation time is 25min;
[0038] Step B: Take the filter residue obtained in step A and disodium hydrogen phosphate-citric acid buffer solution as 1g:30ml, add disodium hydrogen phosphate-citric acid buffer solution to the filter residue obtained in step A, soak at 60°C Extracted for 2 hours, then ultrasonically extracted at a frequency of 300W for 10min, then centrifugally filtered at 4000rpm for 25min to obtain an extract; the disodium hydrogen phosphate-citric acid buffer solution was 5 in pH and contained 4% Cellulase buffer solution, and the activity of ...
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