Method for producing color-changeable light-induced fluorescent spectacular fish

Abstract

The invention provides a method for producing spectacular fish capable of expressing color-changeable light-induced fluorescent light on a whole body and the color-changeable light-induced fluorescent spectacular fish obtained by the method through utilizing a promoter capable of universally starting expression on the whole body to drive the expression of color-changeable light-induced fluorescent light, and further provides a method for converting fishes with many body surface pigments to the spectacular fish capable of expressing the color-changeable light-induced fluorescent light on the whole body by gene modification and a product obtained by the method. Meanwhile, the spectacular fish is sterilized in order to guarantee the bio-security. The invention further provides a method for producing fancy breeds capable of emitting the color-changeable light-induced fluorescent light on the whole body aiming at other aquatic organisms including shrimps and jellyfishes. Therefore, an arousing wavelength is manually changed so that the fish can be changed to the other colors from one color within several seconds to generate an interesting spectacular effect, and furthermore, a pattern of a traditional spectacular fish market is expanded.

Description

technical field [0001] The invention relates to a method for producing fluorescent ornamental fish, in particular to a method for producing fluorescent ornamental fish induced by variable color light. Background technique [0002] Ornamental fish is a fish industry with a global market, and fluorescent fish has greatly changed the ornamental fish market because it can obtain eye-catching colors different from traditional fish, and has a unique decorative effect especially in low light conditions pattern. At present, the use of transgenic technology can obtain fish with specific traits in a short period of time, without the need to slowly change the characteristics of fish through traditional hybridization technology. The use of transgenic technology to obtain fluorescent ornamental fish has achieved great commercial value. [0003] In the prior art, there are many techniques for introducing exogenous genes into fish, including microinjection, virus-mediated gene integratio...

AI Technical Summary

Problems solved by technology

First of all, the existing fluorescent ornamental fish construction technology realizes the tissue-specific expression of fluorescent protein, that is, the fluorescence appears only in specific tissues of the fish, and the fluorescent fish thus constructed lacks a certain degree of ornamental value from the visual point of view.

Secondly, the expression of monochromatic fluorescence is achieved, and the color cannot be changed under external stimuli. The fluorescent ornamental fish obtained from this lacks certain interactivity or interest.

In addition, some fish have more pigmentation on their body surface, which leads to a decrease in the transparency of the fish surface, which not only shields the excitation of the fluorophore by the excitation light, resulting in low luminescence efficiency of the fluorophore, but also blocks the emission of the fluorophore. Light reaches the outside of the body, further reducing ornamental

In order to save this shortcoming to a certain extent, the above-mentioned patent has to use a strong promoter to drive the expression of the fluorescent group, but the price is that the phototoxicity of the fluorescent group affects the health of the fish and greatly shortens the lifespan of the fish

[0005] In addition, none of the fluorescent fish obtained in the prior art has been sterilized. Once such transgenic fish species enter the natural environment, they may cause unpredictable potential harm to natural species

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