A probiotic fermented traditional Chinese medicine compound composition for assisting hypoglycemia and its preparation method and application
A technology of probiotics and compositions, which is applied in the field of probiotic fermented traditional Chinese medicine compound composition for assisting in lowering blood sugar and its preparation and application, can solve the problems of unfavorable diabetes patients for long-term use, lack of coordination, side effects, etc., and achieve conditioning Gastrointestinal function, taste is easy to accept, and the effect of increasing absorption
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Embodiment 1
[0028] Embodiment 1: Extraction method of silkworm chrysalis in probiotic fermented traditional Chinese medicine compound composition
[0029] Take 24g of silkworm chrysalis, dry the washed silkworm chrysalis at 100°C until the water content is less than 5.0%, crush them, pass through a 40-mesh sieve, add 3 times the amount (weight / volume) of silkworm chrysalis powder (weight / volume) 95% ethanol, degrease at 60°C for 2.0 hours, and pump After filtering, dry at 80°C and pass through a 40-mesh sieve to obtain defatted silkworm chrysalis powder. Add 6 times the amount of water (weight / volume) to the defatted silkworm chrysalis powder, soak for 30 minutes, heat and boil for 30 minutes, and cool to 45°C for later use. This is defatted silkworm chrysalis powder. Take 60g of fresh porcine pancreas, remove the fat fascia, mix it with defatted silkworm chrysalis powder after beating, adjust the pH value to 8.0 with sodium hydroxide, enzymatic hydrolysis for about 3 hours, when the pH d...
Embodiment 2
[0030] Example 2 Preparation of probiotic fermented traditional Chinese medicine compound composition
[0031] Grind American ginseng and pass through a 100-mesh sieve to obtain American ginseng powder; weigh various Chinese medicines according to the following weights: 80g kudzu powder, 40g yam, 40g mulberry leaves, 40g medlar, 60g purslane, 25g dried ginger, 100g tartary buckwheat, American ginseng 50g (fine powder), licorice 8g, soak in 8 times of water for 1 hour, extract at 100°C for 1 hour, filter, add 6 times of water to the filter residue, extract at 100°C for 1 hour, filter and combine the filtrates, Add the silkworm chrysalis enzymatic hydrolyzate extracted in Example 1, concentrate under reduced pressure to 1000ml, and obtain the compound Chinese medicine extract. Then add glucose (weight / volume ratio 0.5%), peptone (weight / volume ratio 0.2%), yeast powder (weight / volume ratio 0.1%), yeast extract (weight / volume ratio 0.2%), sucralose (weight / volume ratio 0.017%)...
experiment example 1
[0035] Experimental example 1: The probiotic fermented traditional Chinese medicine compound composition of the present invention assists hypoglycemic and glucose tolerance experiments
[0036] This example aims to use the hyperglycemia model of rats to illustrate the hypoglycemic effect of the probiotic fermented traditional Chinese medicine compound composition prepared according to the method of the present invention.
[0037] Fasting hypoglycemic experiment: the probiotic fermented traditional Chinese medicine compound composition prepared in Example 2 and berberine hydrochloride (Qingdao Huanghai Pharmaceutical Co., Ltd.) were used as test samples. The Johnson & Johnson Blood Glucose Meter measures blood sugar levels.
[0038] Take 80 male rats with a body weight of 180±20g, and feed them adaptively for 3 days, take out 10 rats randomly and feed them alone as the blank control group, and inject streptozotocin into the tail vein of the remaining 70 rats according to the do...
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