Focusing device and focusing method for in vivo microscopic imaging system

Abstract

The invention discloses a focus control device and method for a living body microscopic imaging system. The focus control device comprises an outer pipe, an inner pipe, a microscopic imaging module, a focus fixing cover and a drive motor. The microscopic imaging module is arranged inside the inner pipe, the inner pipe is arranged inside the outer pipe, and the upper end of the inner pipe is connected with the drive motor which is used for driving the inner pipe to move up and down in the outer pipe. The focus control method based on the focus device comprises the following steps that 1, the focus control device for the living body microscopic imaging system is placed above an imaging area, and the focus fixing cover of the focus control device is used for fixing the relative positions of the microscopic imaging module and a living body sample to enable the focus of the imaging module to initially coincide with the live body sample; 2, according to a microscopic image of the microscopic imaging module, the drive motor is controlled to drive the inner pipe to move up and down to adjust the focus of the imaging module, the focus of the imaging module is made to coincide with the living body sample accurately, and therefore a clear microscopic image with high resolution is obtained.

Description

technical field [0001] The invention relates to the field of microscopic imaging systems, in particular to a focusing device and a focusing method for living microscopic imaging systems. Background technique [0002] Microscopic imaging system is an indispensable tool for observation and analysis in the microscopic field of biomedicine. However, the traditional microscopic imaging system needs to cut the biological tissue from the living body first, and then perform a series of sample preparation processes such as wax sealing, dehydration, fixation, staining, and sectioning to obtain microscopic images. [0003] The optical principle of the in vivo microscopic imaging system is the same as that of the traditional microscope, but the sample does not need to go through the above-mentioned complex preparation processes such as sealing wax, dehydration, fixation, staining, and sectioning. The technical key lies in how to adjust the focus so that it coincides with the observatio...

AI Technical Summary

Problems solved by technology

The difficulty in adjusting the focus of the in vivo microscopic imaging system is that the relative position of the living tissue and the in vivo microscopic imaging system is difficult to determine, that is, when the microscope adjusts the focus, the living sample is difficult to fix, which makes it difficult to adjust the focus, resulting in blurred images

Images