Giant salamander mucinous protein, preparation method and applications in cosmetics

A technology for mucin and giant salamander, which is applied in the field of giant salamander mucin and its preparation to achieve the effects of promoting cell growth, improving skin telangiectasia and reducing capillary permeability

Active Publication Date: 2014-03-05
ZHANGJIAJIE JINCHI ANDRIAS DAVIDIANUS BIOLOGICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no report on the preparation of giant salamander mucus protein with the property of agglutinating red blood cells from giant salamander mucus

Method used

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  • Giant salamander mucinous protein, preparation method and applications in cosmetics
  • Giant salamander mucinous protein, preparation method and applications in cosmetics
  • Giant salamander mucinous protein, preparation method and applications in cosmetics

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Experimental program
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Embodiment Construction

[0018] Follow the steps below in turn:

[0019] a. Collect giant salamander mucus, homogenize the giant salamander mucus at 0-10°C, add 1-3 times the volume of 0.01-0.1M Tris-HCl buffer solution with pH 7-8 containing 0.15 M NaCl to the homogenate, place in Centrifuge overnight at 4°C for 20 min at 5000 g, and collect the supernatant;

[0020] b. Add (NH 4 ) 2 SO 4 to 80% saturation concentration, after standing overnight, centrifuge at 5000g for 20 min, collect the precipitate, dialyze and freeze-dry to obtain the freeze-dried powder of giant salamander mucus;

[0021] c. Dissolve 150 mg of giant salamander mucus freeze-dried powder with 50 ml of pH 6-9, 0.01-0.1 M Tris-HCl buffer, and add it to cross-linked ovalbumin-porcine gastric mucin (3:1) affinity chromatography Wash with pH 6-9, 0.01-0.1 M Tris-HCl buffer, then elute with 1.5 M NaCl solution, collect NaCl eluate, dialyze and freeze-dry;

[0022] d. Dissolve the lyophilized powder in pH 6-9, 0.01-0.1 M Tris-HCl fl...

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Abstract

The invention discloses a giant salamander mucinous protein, a preparation method and applications in cosmetics. The giant salamander mucinous protein has a molecular weight of 17000D, contains two subunits with a molecular weight of 8500D. The subunit contains 77 amino acid residues and the N end is valine-glycine-tyrosine-threonine-valine-glycine-alanine-threonine-proline-methionine-. The giant salamander mucinous protein has functions of agglutinating red blood cell activity, reducing capillary permeability, promoting cell growth and the like. Cosmetics prepared from the giant salamander mucinous protein can improve skin telangiectasis (known as couperose skin) effectively.

Description

technical field [0001] The invention relates to a giant salamander mucus protein and a preparation method thereof, in particular to a giant salamander mucus protein with the property of coagulating red blood cells, a preparation method and an application in cosmetics. Background technique [0002] giant salamander ( Andrias davidianus ) is a rare specialty unique to our country and belongs to the second-class national protected animals. It is mainly distributed in streams in the middle and upper reaches of the Yangtze River, the middle and upper reaches of the Pearl River and the upper reaches of the Han River. With the development of breeding scale, the intensive processing and utilization of giant salamander resources has become an urgent issue related to the sustainable development of giant salamander industry. The giant salamander has no scales and multiple skin glands on its body surface, and it constantly secretes mucus during the growth process, which contains a var...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/46C07K1/36C07K1/30C07K1/22A61K8/64A61Q19/00A61P17/00
CPCA61K8/64A61Q19/00C07K14/463
Inventor 李伟佟长青曲敏金桥
Owner ZHANGJIAJIE JINCHI ANDRIAS DAVIDIANUS BIOLOGICAL SCI
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