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Preparation of Ropalidia wasp venom polypeptide and analgesic and anti-inflammatory application thereof

A technology of wasps and uses, which is applied in the field of medicine, can solve the problems of low level of industrial production support and failure to form large-scale production capacity, and achieve the effect of suppressing pain, reducing inflammation and immune inflammatory response

Inactive Publication Date: 2014-03-19
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with developed countries, there is still a relatively obvious gap in the research and development of my country's TTS system, especially the low level of supporting industrial production, which has not yet formed a large-scale production capacity

Method used

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  • Preparation of Ropalidia wasp venom polypeptide and analgesic and anti-inflammatory application thereof
  • Preparation of Ropalidia wasp venom polypeptide and analgesic and anti-inflammatory application thereof
  • Preparation of Ropalidia wasp venom polypeptide and analgesic and anti-inflammatory application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 : Preparation of effective fraction of Vespa venom with bell-bellies and hydrogel

[0047] 1.1 Collection of crude wasp venom with bell-bellies:

[0048] The bell-bellied Vespa was collected from Nalun Township, Guangnan County, Wenshan Prefecture, Yunnan Province. The bee specimen was identified as Ropalidia by Professor Yang Zizhong of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province (hereinafter referred to as: Engineering Research Center) fasciata Fabricius (Vespa bellbellum). Wasp venom collection device: use the auxiliary cover type bee venom collector modified by the engineering research center, wooden frame, 80 cm long, 50 cm wide; embedded with 4 pieces of 20 cm long / 33 cm wide poisonous glass; input voltage 10V , to output automatic intermittent pulsating voltage; the wooden frame is painted red with red paint in advance; the wooden frame is pre-smeared with ...

Embodiment 2

[0053] Example 2 : Preparation of active parts of Vespa venom polypeptide and coating agent

[0054] 2.1 Collection of crude venom from Vespa rufica:

[0055] The wasp was collected from Jinghong County, Yunnan Province. The bee specimen was identified as Ropalidia ferruginea Fabricius (Ropalidia ferruginea Fabricius) by Professor Guo Yunjiao, National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province. . Wasp venom collection device: use the auxiliary cover type bee venom collector modified by the engineering research center, wooden frame, 80 cm long, 50 cm wide; embedded with 4 pieces of 20 cm long / 33 cm wide poisonous glass; input voltage 9V , output automatic intermittent pulsating voltage; method of collecting wasp venom: put the unpainted poison collector in front of the hive, and collect venom from the worker bees of the rusty-edged bell-bellied wasp colony according to the routine poison col...

Embodiment 3

[0060] Example 3 : New bell-bellied Vespa bee venom effective fraction and preparation of cataplasm

[0061] 3.1 Collection of crude venom from New Bell-bellied Vespa:

[0062] The new bell-bellied wasp was collected from Cangyuan, Yunnan Province, and the bee body specimen was identified as Ropalidia artifex Saussure (new bell-bellied wasp) according to Professor Yang Zizhong from the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali College, Yunnan Province. The basic device for poison collection uses a sub-cap type bee venom collector modified by the engineering research center, with a wooden frame, 80 cm long and 50 cm wide; 4 pieces of poisonous glass with a length of 20 cm / width of 33 cm are embedded; the input voltage is 6V, Output automatic intermittent pulsating voltage; add a photodiode bulb bearing layer under the glass plate, use LED bulbs from Zhengzhou Hengguang Technology Co., Ltd., and connect to the b...

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Abstract

The invention relates to preparation of a Ropalidia wasp venom polypeptide and an analgesic and anti-inflammatory application thereof. Specifically, a polypeptide active site is obtained by performing dialysis on crude wasp venom of a Ropalidia wasp by a dialysis membrane with molecular weight cutoff of 15KDa, and performing freeze-drying or decompression concentration drying on an eluate for preparation. A variety of percutaneous absorption preparations of the polypeptide active site can effectively prolong the hot plate-stimulated pain threshold value of mice, obviously inhibit the xylene-induced ear edema of the mice and the egg white-induced toe swelling of the mice, significantly reduce the adjuvant-induced arthritis joint swelling of rats and reduce the joint circumference. The insect polypeptide analgesic and anti-inflammatory active site and the percutaneous absorption preparations can be expected to be used for preparing anti-inflammatory and analgesic biochemical medicaments for treating acute and chronic inflammation, relieving pain before and after various operations and relieving pain and neuropathic pain accompanied by cosmetology, microscopic examination, burns and scalds, cancer pain and other acute and chronic pain.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular, the invention relates to a method for preparing an analgesic and anti-inflammatory effective part from insects of the genus Ropalidia spp. and its medical application. The effective part is prepared from the crude bee venom of the genus Bell-bellied Vespa through a modern extraction process. Various transdermal absorption preparations of the effective part have significant effects on analgesia, anti-acute and chronic inflammation and autoimmune inflammation. It can be expected that the The effective part and its transdermal absorption preparation are used to prepare anti-inflammatory and analgesic biochemical drugs for the treatment of acute and chronic inflammation, as well as for pain relief in various operations, beauty treatment, microscopic examination, pain associated with burns, neuropathic pain and cancer Pain and other acute and chronic pain treatment. The anti-inflammator...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61P25/04A61P29/00
Inventor 吴俊珠郭美仙刘熙郭娜娜赵昱约阿施·史托克希特
Owner DALI UNIV
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