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Application of Schisandra sphenanthera Rehd.et Wils. extract in preparation of medicament for treating liver injury caused by acetaminophen

A technology of Schisandra chinensis and paracetamol, applied in the field of medicine

Inactive Publication Date: 2014-03-19
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on the treatment of liver injury caused by APAP with the extract of Schisandra chinensis.

Method used

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  • Application of Schisandra sphenanthera Rehd.et Wils. extract in preparation of medicament for treating liver injury caused by acetaminophen
  • Application of Schisandra sphenanthera Rehd.et Wils. extract in preparation of medicament for treating liver injury caused by acetaminophen
  • Application of Schisandra sphenanthera Rehd.et Wils. extract in preparation of medicament for treating liver injury caused by acetaminophen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Establishment of fingerprints of Schisandra chinensis extract

[0033] The chemical fingerprint of the extract of Schisandra sinensis was established by high-phase liquid chromatography, and the content of the main components was determined, which was used to calculate the dosage of Schisandra sinensis in the following animal experiments.

[0034] (1) Preparation of Schisandra chinensis extract:

[0035] Take 100 g of Schisandra chinensis, add 8 times the amount of water to decoct for 1 h, filter, discard the filtrate, dry the dregs and crush them, extract twice with 8 times the amount of 70% ethanol, 1.5 h each time, filter, Combine the filtrates and concentrate to obtain the extract of Schisandra chinensis extract.

[0036] (2) Preparation of samples for fingerprint analysis:

[0037] Take 0.5 g of the extract of Schisandra chinensis extract, weigh it accurately, dissolve it in ethanol, ultrasonicate for 60 minutes, add ethanol to make up to 10 ml, filte...

Embodiment 2

[0041] Example 2 The test of Schisandra chinensis extract in the treatment of liver injury caused by paracetamol

[0042] (1) Animal model

[0043] Male C57BL / 6 mice, 6-8 weeks old, were provided by the Experimental Animal Center of Sun Yat-sen University (animal certificate number SCXK (Guangdong) 2011-0029). Raised under standard experimental conditions: temperature (23±2°C), humidity (55±10%), 12-hour light-12-hour dark cycle, free access to water and food. Mice were randomly divided into 5 groups, namely blank control group (normal saline), Schisandra chinensis extract group (calculated as Schisandra A, 9.2 mg / kg, p.o.), APAP group (400 mg / kg, i.p.), APAP and Schisandra chinensis extract combined group (APAP pretreatment, given schisandra chinensis extract containing 9.2 mg / kg schisandra ester A after 4 h, p.o.), APAP and NAC combined group (APAP pretreatment, given 200 mg / kg after 4 h NAC, p.o.). The mice were sacrificed at 12 and 24 hours after the administration of S...

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Abstract

The invention discloses an application of a Schisandra sphenanthera Rehd.et Wils. extract in preparation of a medicament for treating a liver injury caused by acetaminophen (APAP). The main ingredients of the Schisandra sphenanthera Rehd.et Wils extract are schisantherin and deoxyschizandrin. Experiments find that the Schisandra sphenanthera Rehd.et Wils. extract can significantly resist serum ALT (alanine aminotransferase)and AST (aspartate amino transferase)level rise and necrosis of liver cells caused by the liver injury caused by APAP, and inhibits consumption of reductive glutathione in the liver cells, so as to relieve the liver injury degree caused by the APAP. The mechanism relates to activation of an Nrf2-ARE (NF-E2-related factor 2-antioxidative responsive element) signal channel, is manifested by raising mRNA (messenger Ribonucleic Acid) expression of Nrf2, facilitating Nrf2 nuclear translocation, starting a downstream medicament-metabolizing enzyme and transcribing an antioxidant gene. The experiment result shows that the Schisandra sphenanthera Rehd.et Wils. extract has a treatment effect on the liver injury caused by the APAP, and novel method and means are provided for clinical prevention and treatment of the liver injury caused by the APAP.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of an extract of Schisandra chinensis in the preparation of medicine for treating liver injury caused by paracetamol. Background technique [0002] Acetaminophen (APAP), also known as acetaminophen, is currently the most widely used OTC antipyretic and analgesic drug in the world. At present, there are more than 100 kinds of cold medicines and analgesics on the market that contain APAP. Just take the common medicines in China as examples, Tylenol, Baifuning, Baijiahei, Gankang, Crack, 999 Ganmaoling, Bili Tongtong, Kangbid, Suxiaoshangfeng Capsules, Tylenol, Radifer, Vitamin C Yinqiao Tablets, etc. all contain this ingredient. [0003] APAP is safe and effective in therapeutic doses, but overdose can cause liver damage, acute liver failure (ALF) and even death. In the United States, the United Kingdom, Australia and other European and American count...

Claims

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Application Information

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IPC IPC(8): A61K36/57A61K9/48A61K9/16A61K9/14A61K9/08A61K9/107A61K9/10A61P1/16
Inventor 毕惠嫦黄民范晓梅姜伊鸣王莹陈攀
Owner SUN YAT SEN UNIV
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