Fructan extract, and preparation method and application thereof

A technology of extract and fructan, which is applied in the field of preparation of fructan extract, can solve the problems of high cost, time-consuming, and inability to adapt to industrial production, and achieve short time-consuming, low cost, and animal fat reduction The effect of content

Inactive Publication Date: 2014-03-26
SHANGHAI ZHANGJIANG ENG RES CENT OF MODERN PREPARATION TECH OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current preparation methods for preparing fructan from Ophiopogon japonicus have defects such as high cost, time-consuming, and inability to adapt to industrial production, and cannot

Method used

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  • Fructan extract, and preparation method and application thereof
  • Fructan extract, and preparation method and application thereof
  • Fructan extract, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0026] Embodiment 1 (preparation of fructan extract)

[0027] Take 20kg of dried Radix Ophiopogon japonicus (Sichuan Ophiopogon japonicus) root tuber, extract twice with 4 times the weight of 90% ethanol under reflux, discard the extract; extract twice with 10 times the amount of water, combine the water extract, and extract The solution was concentrated under reduced pressure to 1.0g / ml; the concentrated solution was added to 3 times the volume of 95% ethanol for precipitation, left to stand, and the precipitate was collected; the obtained supernatant was repeatedly precipitated by alcohol once, and the precipitate obtained by alcohol precipitation was combined twice. Freeze-drying is the fructan extract with a molecular weight in the range of 2500-8000. Get 4.62kg of fructan extract, the yield is: 15.4%. The structure was identified as fructan.

Example Embodiment

[0028] Embodiment 2 (preparation of fructan extract)

[0029] Take 30kg of dried Radix Ophiopogon japonicus (Sichuan Ophiopogon japonicus) tuber root, reflux extraction with 8 times the weight of 70% ethanol for 3 times, and discard the extract; The solution was concentrated under reduced pressure to 0.8g / ml; the concentrated solution of water extraction was precipitated by adding 5 times the volume of 80% ethanol for precipitation, left to stand, and the precipitate was collected; the obtained supernatant was repeatedly precipitated by alcohol once, and the precipitate obtained by alcohol precipitation was combined twice. Freeze-drying is the fructan extract with a molecular weight in the range of 2500-8000. Get 3.15kg of fructan extract, yield: 10.5%. The structure was identified as fructan.

Example Embodiment

[0030] Embodiment 3 (preparation of fructan extract)

[0031] Take 30kg of dried Radix Ophiopogon japonicus (Sichuan Ophiopogon japonicus) root tubers, extract once with 2 times the weight of 100% ethanol under reflux, and discard the extract; The solution was concentrated under reduced pressure to 1.2g / ml; the concentrated solution was added to 1 volume of 100% ethanol for precipitation, left to stand, and the precipitate was collected; the obtained supernatant was repeated alcohol precipitation once, and the precipitate obtained by alcohol precipitation was combined twice. Freeze-drying is the fructan extract with a molecular weight in the range of 2500-8000. Get 3.03kg of fructan extract, yield: 10.1%. The structure was identified as fructan.

[0032] Known from embodiment 1-3, the yield of the fructan extract of embodiment 1 is the highest, therefore embodiment 1 is a preferred embodiment.

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Abstract

The invention discloses a fructan extract and a preparation method thereof. The preparation method comprises the following steps: extracting Sichuan ophiopogon root tuber with ethanol to remove impurities, extracting with water, carrying out ethanol fractional precipitation, and drying to obtain a fructan extract (the molecular weight is 2500-8000). The method for preparing the fructan extract has the advantages of short time consumption and low cost, and can perform abundant preparation and adapt to industrial production. Besides, the invention also discloses application of the fructan extract in preparing medicines, foods or health products for losing weight. The inspection on functions of the fructan extract by a diet-induced fat mouse model proves that the fructan extract has an obvious action of losing weight.

Description

technical field [0001] The invention relates to a fructan extract, and also relates to a preparation method and application of the fructan extract. Background technique [0002] Fructan is a general term for a class of carbohydrates that are ubiquitous in nature and are mainly connected by glycosidic bonds of fructosyl fructose; each fructan molecule contains a molecule of glucose, and its molecular terminal is not reduced. sex. The structure of fructan is quite complicated according to the difference in the position of fructosyl transfer connection and the degree of polymerization among species. Because fructan basically does not decompose and is not digested and absorbed in the process of human mouth, stomach and small intestine. Therefore, it has better weight loss, blood sugar regulation, blood lipid lowering, prevention of heart and brain diseases; improvement of intestinal function, prevention of constipation and diarrhea; promotion of mineral absorption and synthesi...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/733A61P3/04A23L1/09A23L33/125
Inventor 阮克锋冯怡从文娟王源林晓
Owner SHANGHAI ZHANGJIANG ENG RES CENT OF MODERN PREPARATION TECH OF TRADITIONAL CHINESE MEDICINE
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