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A method for improving the phosphorus uptake rate of rice

An absorption rate, rice technology, applied in the field of plant genetic engineering, can solve problems such as waste of phosphorus fertilizer resources and environmental pollution, and achieve the effects of reducing the application of phosphorus fertilizer, increasing the total surface area of ​​roots, and increasing the total number of roots

Active Publication Date: 2015-09-30
BIOLOGICAL TECH INST OF FUJIAN ACADEMY OF AGRI SCI
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Problems solved by technology

In addition, due to the physical and chemical characteristics of the soil, the utilization rate of phosphate fertilizer in the current season is only 10% to 25%. Excessive application of phosphate fertilizer has brought problems such as waste of resources and environmental pollution.

Method used

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  • A method for improving the phosphorus uptake rate of rice
  • A method for improving the phosphorus uptake rate of rice
  • A method for improving the phosphorus uptake rate of rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 3

[0062] Example 3. Transgenic rice tolerance to low phosphorus experiment

[0063] 1. Low-phosphorus culture of transgenic rice

[0064] The homozygous transgenic T4 rice was used as the material, and the non-transgenic pair Guangminghui 86 was used as the control. Take plump rice seeds, soak them at room temperature, put them in a 30°C incubator for 2 days to accelerate germination after 2 days, and after the seeds germinate, move the seedlings into medium containing 1 / 2 MS for 5 days. The seedlings with a height of about 5 cm are moved into a soilless culture box with quartz sand as a matrix containing liquid medium with different phosphorus concentrations for cultivation. The cultivation box is placed in a rainproof, ventilated and sunny plastic greenhouse. The quartz sand was pre-soaked with dilute hydrochloric acid for 5 days, and then rinsed with deionized water several times to wash off the hydrochloric acid and measure the pH value. During the cultivation period, th...

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Abstract

The invention discloses a method for increasing the absorption rate of rice to phosphorus. The method comprises the steps of cloning the overall length cDNA (Complementary Deoxyribonucleic Acid) of an OsSUT2 gene by using a PCR (Polymerase Chain Reaction) technology; constructing a plant expression vector, wherein the plant expression vector is driven by a corn Ubi promoter; obtaining over-expressed OsSUT2 gene rice by using an agrobacterium-mediated method. Transgenic homozygous rice has the advantages that the absorption rate of plants to phosphorus is remarkably increased under a low-phosphorus growth condition, the plant height, tiller number, leaf number and spike number are remarkably increased, and the grain number per spike and the maturing rate also trends to be increased. The invention develops the novel application of the OsSUT2 gene.

Description

technical field [0001] The invention relates to plant genetic engineering, in particular to the application of a rice sucrose transport gene OsSUT2 in improving phosphorus absorption of rice. Background technique [0002] Phosphorus is one of the most important elements for plant growth and development. Because most of the phosphorus in the soil exists in the form of ineffective phosphorus that is difficult for plants to absorb and utilize, the available phosphorus in 30-40% of the cultivated land in the world cannot meet the needs of normal growth and development of plants. Phosphorus deficiency is common in crops. In addition, due to the physical and chemical characteristics of the soil, the utilization rate of phosphate fertilizer in the current season is only 10% to 25%. Excessive application of phosphate fertilizer has brought problems such as waste of resources and environmental pollution. Therefore, cultivating crop varieties that efficiently utilize phosphorus fert...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/84C12N15/66
Inventor 苏军张武君宋亚娜付艳萍颜静宛胡太蛟李刚林智敏
Owner BIOLOGICAL TECH INST OF FUJIAN ACADEMY OF AGRI SCI
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