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Test strip and method for detecting spectinomycin

A spectinomycin, test strip technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of drug residue pollution, damage to environmental balance, allergic reactions, etc., achieve low specificity and affinity, increase reaction sensitivity, Sensitive effect

Inactive Publication Date: 2014-04-09
BEIJING KWINBON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some unscrupulous traders are driven by economic interests to abuse antibiotics, leaving a large amount of antibiotics in animal products. If you eat animal-derived foods containing antibiotic residues for a long time, it is equivalent to absorbing antibiotics in low doses for a long time, which will cause antibiotic resistance in the human body. , will bring adverse effects to the use of antibiotics when sick, will destroy the balance of the internal environment of the body, cause flora imbalance, people with antibiotic allergies will have allergic reactions, cause immune hemolysis, and cause drug residue pollution in food and the environment at the same time series of questions

Method used

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  • Test strip and method for detecting spectinomycin
  • Test strip and method for detecting spectinomycin
  • Test strip and method for detecting spectinomycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, detect the composition of the test paper strip of Spectinomycin

[0050] 1. Freeze-dried microwell strips with gold-labeled antibodies

[0051] Microporous reagents are housed in the microwell strip, wherein 1 is a microwell strip, and 2 is a microporous reagent of freeze-dried spectinomycin specific antibody-colloidal gold marker;

[0052] The microwell strip containing the micropore reagent has a microwell plug 3 ( figure 2 with image 3 );

[0053] The micropore reagent of the lyophilized spectinomycin monoclonal antibody-colloidal gold marker in the microwell strip, the lyophilized amount of the spectinomycin specific antibody-colloidal gold marker is 0.20-0.25 μg / ml.

[0054] Two, test paper ( Figure 4 with Figure 5 )

[0055] The test paper is composed of a bottom plate, a sample absorption pad, a reaction film, a water absorption pad, and a protective film;

[0056] The sample absorbent pad 4, reaction film 5, water absorbent pad 6 and pro...

Embodiment 2

[0061] The preparation method of test strip described in embodiment 2, embodiment 1

[0062] 1. Preparation of test strips

[0063] The preparation method of this test strip mainly comprises the steps:

[0064] 1) Prepare freeze-dried spectinomycin monoclonal antibody-colloidal gold-labeled microwell reagent in a microwell strip, and cover the microwell strip with a microwell plug.

[0065] 2) Prepare a reaction membrane with a detection line (T) coated with a spectinomycin-carrier protein conjugate and a quality control line (C) coated with a goat anti-mouse anti-antibody;

[0066] 3) Assemble the reaction film prepared in 2) with the sample absorbent pad, water absorbent pad, protective film, and bottom plate to form a test paper;

[0067] 4) Assemble the freeze-dried microwell reagent, test paper, microwell strip and microwell plug prepared in 1) and 3) with spectinomycin monoclonal antibody-colloidal gold marker into a test strip.

[0068] The following step-by-step det...

Embodiment 3

[0116] Embodiment 3, the application of test strip

[0117] One, detect Spectinomycin in milk with the test strip described in Example 1

[0118] The test strip of the invention can detect milk samples. The general detection method is as follows:

[0119] 1. Detection method

[0120] Remove the microwell plug covered on the microwell strip, and drop the sample solution to be detected into the microwell strip containing the microwell reagent to obtain a mixed solution 11 of the microwell reagent and sample solution, mix well, and incubate 5min, insert the end of the test strip 12 with the MAX line mark down into the microwell strip, and watch the result within 5min, as Figure 7 shown.

[0121] 2. Judgment of test results

[0122] Positive: When the quality control line (C) shows a red band, but the test line (T) does not show color, and the test paper shows a red line "|", it is judged as positive, such as Figure 8 .a shown.

[0123] Negative: When the quality control ...

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Abstract

The invention discloses a test strip and a method for detecting spectinomycin. The test strip comprises a micropore strip, a micropore reagent, test paper and a micropore plug, wherein the micropore reagent is a spectinomycin specific antibody marked by freeze-dried colloidal gold, and the spectinomycin specific antibody is a monoclonal antibody; and the test paper is composed of a sample absorption cushion, a reaction film, a water absorption cushion, a protection film and a bottom plate which are connected in order, the reaction film comprises a strip of detection line print and a strip of quality control line print, the detection line is coated with spectinomycin-carrier protein conjugate, and the quality control line is coated with an antiantibody. The method for detecting spectinomycin by the test strip has advantages of simpleness, rapidness, perceptual intuition, accuracy, convenience for carrying, wide suitable range, low cost, and easy popularization and usage.

Description

technical field [0001] The invention relates to a test strip and a method for detecting spectinomycin, in particular to a test strip and a method for detecting spectinomycin in milk. Background technique [0002] Spectinomycin, also known as spectinomycin, spectinomycin, spectinomycin, etc., is an aminocycloalcohol antibiotic produced by Streptomyces grandis, which is effective against Gram-negative bacteria (such as Brucella, Kray Bacteria, Proteus, Salmonella, Pasteurella, etc.) have strong effects on Gram-positive bacteria (Streptococcus, Staphylococcus, etc.), and have certain effects on Mycoplasma. It is a new type of special treatment Antibiotics for gonorrhea, which have a strong antibacterial effect on Neisseria gonorrhoeae. [0003] It can promote animal growth and is a good animal feed additive. It is widely used in animal husbandry as veterinary medicine. Some unscrupulous traders are driven by economic interests to abuse antibiotics, leaving a large amount of a...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/577G01N33/558
Inventor 冯才伟孙震罗晓琴刘叶赵正苗韩京朋陶光灿朱亮亮
Owner BEIJING KWINBON BIOTECH
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