Salviae miltiorrhizae artificial cultivation method

A technology of artificial cultivation and salvia miltiorrhiza, applied in cultivation, botany equipment and methods, seed and rhizome treatment, etc., can solve the problems of quality and yield decline, unstable content of active ingredients, low germination rate of salvia miltiorrhiza seeds, etc., to promote germination Effect

Inactive Publication Date: 2014-04-23
SHAANXI NORMAL UNIV
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Problems solved by technology

The demand for Salvia miltiorrhiza is gradually increasing, and wild resources cannot meet the demand. Artificial cultivation has become the fundamental way to solve the source of medicine.
The propagation methods of salvia miltiorrhiza include seed propagation, split root propagation, cutting propagation and reed head propagation, etc., because split root propagation, cutting propagation and reed head propagation can shorten the growth cycle of salvia miltiorrhiza and are commonly used by people. , the content of active ingredients is extremely unstable, viral diseases and other phenomena lead to a decline in quality and output, which seriou

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Embodiment Construction

[0029] The present invention will be described in detail below in conjunction with the accompanying drawings.

[0030] In the previous work of the present invention, an endophytic fungus TR21 producing tanshinone ⅡA was isolated, and three strains of endophytic fungi capable of high-yield tanshinone ⅡA were induced by mutagenesis using TR21 as the starting strain (see Document 1). The purpose of this study is to observe the effects of these four strains on the growth of Salvia miltiorrhiza, and solve the problems of artificially cultivated salvia miltiorrhiza from the perspective of endogenous sources.

[0031] Literature (1) Caixia Ma, Dongliang Jiang, Xiying Wei. Mutation breeding of Emericella foeniculicola TR21 for improved production of tanshinone IIA. Process Biochemistry, 2011,46(10):2059~2063.

[0032] 1 Materials and methods

[0033] 1.1 Materials

[0034] 1.1.1 Medium

[0035] Potato dextrose agar medium (PDA): 200g potato, 20g glucose, 18g agar, 1000mL distilled ...

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Abstract

The invention provides a salviae miltiorrhizae artificial cultivation method. According to the influences of salviae miltiorrhizae endophytic fungi on growth of host plants, namely, salviae miltiorrhizae, the fungal fermentation liquor seed soaking method is adopted, and the germinative force and germination rate of salviae miltiorrhizae seeds are remarkably higher than those of contrast; through the salviae miltiorrhizae seedling and fungus co-cultivation method, the growth of salviae miltiorrhizae seedlings is promoted; by spraying fungal fermentation liquor, the growth of salviae miltiorrhizae and the quality improvement on salviae miltiorrhizae are promoted, the growth state of artificially-cultivated salviae miltiorrhizae is remarkably improved, and the foundation is laid for artificial plantation and transplantation of salviae miltiorrhizae.

Description

technical field [0001] The invention belongs to the field of cultivation of salvia miltiorrhiza, in particular to an artificial cultivation method of salvia miltiorrhiza. Background technique [0002] Endophytic fungi refer to fungi that exist in healthy plant tissues at a certain stage in their life history and do not cause obvious symptoms or damage to the host. Studies have found that plant endophytic fungi can promote plant growth by improving the nutritional conditions of plants, which is manifested in many aspects such as increasing the total biomass of plants, improving seed germination rate, seedling survival rate and inhibiting pathogenic bacteria; they can also produce secondary metabolism It can quickly activate the host's adversity response system and enhance the host's ecological adaptability to the environment. Endophytic fungi have shown bright application prospects in biopharmaceuticals, agricultural production, industrial fermentation, etc., and have attrac...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01C1/00A01G9/10
Inventor 魏希颖王凤霞马彩霞万陕宁井明博刘颖王喆之
Owner SHAANXI NORMAL UNIV
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