Method for detecting enrofloxacin metabolic products in sea cucumber

A technology of metabolites and sea cucumbers, applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problems of fast analysis speed, high price, high maintenance cost, etc., and achieve the effect of high sensitivity

Active Publication Date: 2014-04-23
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

Time-of-flight mass spectrometry belongs to high-resolution mass spectrometry, which can help the identification of unknown compounds an

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  • Method for detecting enrofloxacin metabolic products in sea cucumber
  • Method for detecting enrofloxacin metabolic products in sea cucumber
  • Method for detecting enrofloxacin metabolic products in sea cucumber

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Embodiment

[0071] Example Determination of metabolites of enrofloxacin in the process of nitrogen deethylation metabolism in sea cucumbers

[0072] This example is a method for analyzing enrofloxacin metabolites in sea cucumbers by using high performance liquid chromatography-tandem mass spectrometry-ion trap mass spectrometry (LC-MS / MS-Qtrap) technology. Sea cucumber samples were dehydrated with anhydrous sodium sulfate, and enrofloxacin and its metabolites in the samples were extracted with acidified acetonitrile. / MS-Qtrap) method to analyze the enrofloxacin metabolites and their structures in sea cucumbers.

[0073] Enrofloxacin has a variety of metabolic pathways in the body and is converted into a variety of metabolites. Under the action of various enzymes in the body, N-oxidation reactions, N-dealkylation reactions, and N-acetylation reactions can occur And chemical reactions such as the degradation of piperazine ring. Studies have shown that the metabolites of enrofloxacin in d...

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Abstract

The invention discloses a method for detecting enrofloxacin metabolic products in a sea cucumber, belonging to the technical field of aquatic product detection. The method comprises the following steps: sample extraction; sample purification; standard curve drawing; and structural analysis of conditions of instruments used in experiments and metabolic products. According to the invention, a liquid chromatography-tandem mass spectrum-ion trap technology is applied, and a pMRM method is adopted, so that the predictable metabolic products can be accurately determined, and low-concentration metabolic products can also be detected; and by adopting the unique MRM-IDA-EPI working mode of QTrap and adopting one-step sample injection, the MRM chromatograms of the corresponding metabolic products can be obtained, and MS2 mass spectrums can also be obtained. The method can be used for detecting 10 kinds of metabolic products including ciprofloxacin, and the mass spectrum response strengths of two kinds of newly found metabolic products are far higher than that of the ciprofloxacin. By using the method, the blank that no method for detecting the enrofloxacin metabolic products in the sea cucumber is provided at present in China is made up, and a novel reference ideal is provided for researching the metabolic products of other drugs and screening unknown drugs.

Description

technical field [0001] The invention belongs to the technical field of aquatic product detection, and relates to a method for detecting enrofloxacin metabolites in sea cucumbers by using high performance liquid chromatography-tandem mass spectrometry-ion trap mass spectrometry (LC-MS / MS-Qtrap). Background technique [0002] Enrofloxacin (ENR) belongs to the third generation of quinolone synthetic antibacterial drugs and is one of the main antibacterial drugs in aquaculture. The connection function interferes with the synthesis of DNA, RNA and protein, thereby achieving the purpose of antibacterial and bactericidal. However, the non-standard use and abuse of enrofloxacin can be found in animals, food, [0003] Bacterial resistance increases in the environment and in consumers, posing a serious threat to human and animal health. [0004] Enrofloxacin has different degradation or metabolism pathways in the environment and in animals, and its degradation products in the enviro...

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Application Information

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IPC IPC(8): G01N27/62G01N30/02
Inventor 邢丽红孙伟红李兆新翟毓秀郭江涛郭萌萌吴海燕唐锡招
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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