Method for detecting enrofloxacin metabolic products in sea cucumber

Abstract

The invention discloses a method for detecting enrofloxacin metabolic products in a sea cucumber, belonging to the technical field of aquatic product detection. The method comprises the following steps: sample extraction; sample purification; standard curve drawing; and structural analysis of conditions of instruments used in experiments and metabolic products. According to the invention, a liquid chromatography-tandem mass spectrum-ion trap technology is applied, and a pMRM method is adopted, so that the predictable metabolic products can be accurately determined, and low-concentration metabolic products can also be detected; and by adopting the unique MRM-IDA-EPI working mode of QTrap and adopting one-step sample injection, the MRM chromatograms of the corresponding metabolic products can be obtained, and MS2 mass spectrums can also be obtained. The method can be used for detecting 10 kinds of metabolic products including ciprofloxacin, and the mass spectrum response strengths of two kinds of newly found metabolic products are far higher than that of the ciprofloxacin. By using the method, the blank that no method for detecting the enrofloxacin metabolic products in the sea cucumber is provided at present in China is made up, and a novel reference ideal is provided for researching the metabolic products of other drugs and screening unknown drugs.

Description

technical field [0001] The invention belongs to the technical field of aquatic product detection, and relates to a method for detecting enrofloxacin metabolites in sea cucumbers by using high performance liquid chromatography-tandem mass spectrometry-ion trap mass spectrometry (LC-MS / MS-Qtrap). Background technique [0002] Enrofloxacin (ENR) belongs to the third generation of quinolone synthetic antibacterial drugs and is one of the main antibacterial drugs in aquaculture. The connection function interferes with the synthesis of DNA, RNA and protein, thereby achieving the purpose of antibacterial and bactericidal. However, the non-standard use and abuse of enrofloxacin can be found in animals, food, [0003] Bacterial resistance increases in the environment and in consumers, posing a serious threat to human and animal health. [0004] Enrofloxacin has different degradation or metabolism pathways in the environment and in animals, and its degradation products in the enviro...

AI Technical Summary

Problems solved by technology

Time-of-flight mass spectrometry belongs to high-resolution mass spectrometry, which can help the identification of unknown compounds and the distinction of m / z approximate ions. The analysis speed is fast, but it is expensive, more precise, and has high maintenance costs.

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