Application of compound microorganism bacterium agent in treatment of petrochemical sewage and sludge
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A technology of compound bacterial agent and petrochemical sewage, applied in the field of microorganisms, can solve the problems of few and few effective oil-degrading bacteria, etc.
Inactive Publication Date: 2014-04-30
LANZHOU PETROCHEM COLLEGE OF VOCATIONAL TECH
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[0002] At present, there are not many studies on the use of microorganisms to treat petrochemical sewage sludge in industrial wastewater, and there are fewer effective oil-degrading bacteria that can be used for water treatment.
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Embodiment 1
[0029] The method of treating petrochemical sewage sludge with microbial compound agent is as follows:
[0030] 1. Preparation of biological carrier: the pore size is 250μm, the surface area is 60m 2 Add 50ml 5% glutaraldehyde to 1g polyphenylene oxide / g, suspend and stir for 48 hours, wash with 2,4-dinitrohydrazine, wash with water, add 0.75mol / L CaCl 2 The 50mmol / L PBS (pH5.7) buffer solution was allowed to stand at room temperature for 12 hours; it made the microorganisms easy to multiply and survive in the conjunctiva, that is, the microorganisms were easy to fix;
[0031] 2. Spread the microbial compound bacterial agent on the LB solid medium, shake and culture it on a shaker at 30°C and 150rpm / min for 24-48 hours, until the concentration of the microbial compound bacterial agent is 10 3 a / ml;
[0032] 3. Put 50ml of microbial compound suspension prepared in step (2) into 6g of biological carrier prepared in step (1), stir at 20°C for 30min, and let stand at 4°C for 48 ...
Embodiment 2
[0035] The method of treating petrochemical sewage sludge with microbial compound agent is as follows:
[0036] 1. Preparation of biological carrier: the pore size is 200μm, the surface area is 70m 2 Add 50ml 5% glutaraldehyde to 1g polyphenylene oxide / g, suspend and stir for 48 hours, wash with 2,4-dinitrohydrazine, wash with water, add 0.75mol / L CaCl 2 The 50mmol / L PBS (pH5.7) buffer solution was allowed to stand at room temperature for 12 hours; it made the microorganisms easy to multiply and survive in the conjunctiva, that is, the microorganisms were easy to fix;
[0037] 2. Spread the microbial compound bacterial agent on the LB solid medium, shake and cultivate it on a shaker at 20°C and 150rpm / min for 24-48 hours, until the concentration of the microbial compound bacterial agent is 10 3 a / ml;
[0038] 3. Put 50ml of microbial composite bacterial agent suspension prepared in step (2) into 6g of biological carrier prepared in step (1), stir at 25°C for 30min, and stand...
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Abstract
The invention provides an application of a compound microorganism bacterium agent in treatment of petrochemical sewage and sludge. The application comprises the following steps: (1) preparing a biological carrier, to be specific, adding glutaraldehyde to polyphenylene oxide, suspending, stirring, washing, then adding a PBS (Phosphate Buffer Solution) containing CaCl2 and standing; (2) carrying out enlarging cultivation of the compound microorganism bacterium agent; (3) putting the compound microorganism bacterium agent subjected to enlarging cultivation, obtained in the step (2), in the biological carrier prepared in the step (1); and (4) adding the biological carrier loaded with the compound microorganism bacterium agent, obtained in the step (3), to the petrochemical sewage sludge, wherein the compound microorganism bacterium agent continuously increases to a stable period and forms a biological membrane on the surface of the biological carrier, and the biological membrane metabolizes, adsorbs, absorbs, digests and resolves organic pollutants and heavy metals in petrochemical sewage and sludge, so that the petrochemical sewage and sludge are converted into stable harmless materials. Through research, the applicant finds that the compound microorganism bacterium agent can be used for treating the petrochemical sewage and sludge very well.
Description
technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to the application of a microbial composite bacterial agent in treating petrochemical sewage sludge. Background technique [0002] At present, there are not many studies on the use of microorganisms to treat petrochemical sewage sludge in industrial wastewater, and there are even fewer effective petroleum-degrading bacteria that can be used for water treatment. Lu Xianyan et al. isolated 2 strains from the wastewater of Guangzhou Petrochemical Sewage Treatment Plant, and used self-prepared oily wastewater as experimental water. After 7 days of treatment, the COD removal rates of the 2 strains were 70% and 50% respectively; the highest COD The removal rates were 83.67% and 57.49%. Contents of the invention [0003] The invention provides the application of a microbial composite bacterial agent in treating petrochemical sewage sludge, which can well treat petroch...
Claims
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Application Information
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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F11/02C02F101/32
CPCC02F3/34C02F2101/20C02F2101/30C02F2103/365
Inventor 张满效谢小冬张威陈拓刘光琇
Owner LANZHOU PETROCHEM COLLEGE OF VOCATIONAL TECH
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