Method for building nucleotide formula to identify plant variety by using ribosome deoxyribonucleic acid (DNA)

Abstract

The invention discloses a method for identifying plant variety, and provides a method for identifying crape myrtle variety. The method comprises the following steps: 1) carrying out polymerase chain reaction (PCR) amplification by taking a genome deoxyribonucleic acid (DNA) of to-be-detected crape myrtle as a template and using a primer composed of two single-chain DNA molecules shown in a sequence 1 and a sequence 2, so as to obtain a PCR product; 2) sequencing the PCR product, and listing the nucleotide formulas of the to-be-detected crape myrtle according to the sequencing result; 3) comparing the nucleotide formulas obtained in the step 2) with corresponding nucleotide formulas of a plurality of crape myrtle varieties, so as to determine the variety of the to-be-detected crape myrtle. An experiment proves that the method is accurate, and simple and fast to operate, the production efficiency and the quality monitoring level of related enterprises can be improved, and the defects of the traditional method are effectively compensated. In addition, identification of a non-flowering phase (for example, a seedling stage) is simultaneously achieved. The method has important significance on facilitation of development of new variety breeding of the crape myrtle, crape myrtle flower and wood industries and urban garden greening, and environmental protection.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for identifying or assisting in identifying plant varieties, in particular to a method for identifying plant varieties by using ribosomal DNA sequences to construct nucleotide molecular formulas. Background technique [0002] Plant varieties refer to cultivated plants that are formed through artificial breeding with basically the same species, relatively stable heredity, and certain economic traits that humans need: such as ornamental traits, medicinal traits, or other economic traits, and are used as special means of production. group. Such as peony varieties, walnut varieties, apple varieties, crape myrtle varieties, etc. [0003] Plant varieties are generally identified and identified using phenotypic traits. However, there are seasonal differences in the performance of plant varieties, and in some seasons, there may be no traits that differ between varieties. For exampl...

AI Technical Summary

Problems solved by technology

However, due to the lack of sorting of variety resources, the production, promotion and breeding of Lagerstroemia indica varieties are limited.

The sources of traditional crape myrtle varieties are not well documented, and there are phenomena of the same thing with different names and different names with the same name, etc., which lead to irregularities in the market and affect the normal trade of crape myrtle flowers and trees and the growth of the economic scale of related industries.

The lack of morphological markers and DNA molecular markers and the lack of a reliable identification technology system seriously restrict the genetic evaluation, protection and utilization of Lagerstroemia germplasm resources

Images