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Culture medium optimizing and short cut log cultivating method of phellinus igniarius

A cultivation method and short-segment wood technology, which are applied in the field of Phellinus japonica culture substrate optimization and short-segment wood cultivation, to achieve rich economic returns, make up for the effects of species resources being on the verge of depletion, and high content of active ingredients

Active Publication Date: 2014-05-21
通化长白山药谷集团有限公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cultivation of artificial wood segments has just started, and there is no specific report on the cultivation of Phellinus chinensis by artificial wood segments, and there is no domestic harvest of the finished Phellinus chinensis fruiting bodies cultivated artificially.

Method used

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  • Culture medium optimizing and short cut log cultivating method of phellinus igniarius
  • Culture medium optimizing and short cut log cultivating method of phellinus igniarius
  • Culture medium optimizing and short cut log cultivating method of phellinus igniarius

Examples

Experimental program
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Effect test

experiment example 1

[0026] Study on the Culture Conditions of Phellinus Phellinus Mycelium

[0027] 1 Materials and methods

[0028] 1.1 Phellinus strain (Phellinus igniarius), collected from the oak (Quercus mongolica) in the old mountain forest of the Changbai Mountains.

[0029] 1.2 Test medium

[0030] Basal medium: glucose 20g, soybean peptone 2g, agar 18g, potassium dihydrogen phosphate 0.46g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, water 1000ml.

[0031] 1.3 Experimental method

[0032] 1.3.1 Isolation and purification of strains

[0033] Phellinus parent species were isolated by fruiting body tissue separation method. Under strict aseptic operation conditions, the surface of the wild Phellinus radix fruiting body collected from Changbai Mountain was disinfected, the surface of the fruiting body was repeatedly wiped with 75% alcohol, and then placed in a sterile flat petri dish, and then the petri dish was placed in an ultra-clean Standby in the workbench. Use ...

experiment example 2

[0064] Study on Culture Substrate of Phellinus Phellinus Original Seeds and Cultivated Seeds

[0065] The production of Phellinus fungus strains is a very important part in the whole Phellinus cultivation process. Only excellent strains, coupled with good cultivation conditions, can successfully obtain the fruiting body of Phellinus japonica. In addition to the type of strain, the key to obtaining excellent strains is the selection of culture medium or culture substrate. Only with a suitable culture substrate can strong and vigorous strains be obtained. This experiment started from three aspects: the selection of mother seed medium, the original seed medium selection, and the selection of cultivar culture material, in order to obtain excellent Phellinus fungus strains and provide prerequisite guarantee for the successful cultivation of Phellinus fungus fruiting bodies.

[0066] 1 Screening of the original culture medium of Phellinus fungus

[0067] 1.1 Materials and methods ...

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Abstract

The invention relates to cultivation of a fungus plant, and relates to a culture medium optimizing and short cut log cultivating method of phellinus igniarius. The phellinus igniarius stock culture medium comprises 20g of glucose, 2g of soy peptone, 18g of agar, 0.46g of monopotassium phosphate, 1g of dipotassium phosphate, 0.5g of magnesium sulfate and 1000ml of water. The short cut log cultivating method of phellinus igniarius comprises the following steps of (1) separating phellinus igniarius stock species by using a substantive tissue separation method, (2) culturing phellinus igniarius stock species, (3) culturing phellinus igniarius cultivated species, and (4) preparing short cut log cultivation material. Chinese oak logs are selected as bacteria sticks, so that the cost of artificially cultivating phellinus igniarius is low, the yield is high, the quality is good, the content of effective elements is high and abundant economic returns can be obtained.

Description

[0001] technical field [0002] The invention relates to the cultivation of fungus plants, that is, the optimization of the culture substrate of Phellinus japonica and the cultivation method of short-segment wood. Background technique [0003] In the prior art, Phellinus is a rare large-scale medicinal fungus, and its medicinal value has been recorded as early as in the "On the Properties of Medicine" in the Tang Dynasty. "Compendium of Materia Medica" records that Phellinus can cure addiction and drink accumulation, abdominal pain and sores; "Dictionary of Chinese Medicine" describes that it can treat various diseases in internal medicine. Its fruiting body is used as medicine, with a slightly bitter taste, which can benefit the five internal organs, relieve intestinal qi, stop bleeding, detoxify, harmonize the stomach and stop diarrhea. Modern medical research shows that Phellinus not only has the theoretical pharmacological effects of traditional Chinese medicine, but also...

Claims

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Application Information

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IPC IPC(8): C05G1/00A01G1/04
Inventor 李喜范李春娥杨坤杨静
Owner 通化长白山药谷集团有限公司
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