Culture medium optimizing and short cut log cultivating method of phellinus igniarius

A cultivation method and short-segment wood technology, which are applied in the field of Phellinus japonica culture substrate optimization and short-segment wood cultivation, to achieve rich economic returns, make up for the effects of species resources being on the verge of depletion, and high content of active ingredients

Active Publication Date: 2014-05-21
通化长白山药谷集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cultivation of artificial wood segments has just started, and there is no specific report on the cultivation of Phellinus chinensis b

Method used

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  • Culture medium optimizing and short cut log cultivating method of phellinus igniarius
  • Culture medium optimizing and short cut log cultivating method of phellinus igniarius
  • Culture medium optimizing and short cut log cultivating method of phellinus igniarius

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Experimental program
Comparison scheme
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Example Embodiment

[0025] Experimental example 1

[0026] Study on the Culture Condition of Phellinus igniarius Hyphae

[0027] 1Materials and methods

[0028] 1.1 Phellinus igniarius strain (Phellinus igniarius), collected from oak wood (Quercus mongolica) in the forest of the aged mountains of the Changbai Mountains.

[0029] 1.2 Test medium

[0030] Basic medium: 20g glucose, 2g soy peptone, 18g agar, 0.46g potassium dihydrogen phosphate, 1g dipotassium hydrogen phosphate, 0.5g magnesium sulfate, 1000ml water.

[0031] 1.3 Experimental method

[0032] 1.3.1 Strain isolation and purification culture

[0033] The mother species of Phellinus linteus were separated by the method of separating fruit body tissue. Under strict aseptic operation conditions, perform surface disinfection treatment on the fruit bodies of wild Phellinus igniarius from Changbai Mountain. Repeatedly rub the surface of the fruit bodies with 75% alcohol and place them in a sterile flat petri dish, and then put the petri dish into a ...

Example Embodiment

[0063] Experimental example 2

[0064] Study on the culture quality of the original and cultivated species of Phellinus igniarius

[0065] The production of Phellinus igniarius strains is a very important part of the entire cultivation process of Phellinus igniarius. Only with fine strains and good cultivation conditions can it be possible to successfully obtain Phellinus igniarius fruiting bodies. In addition to the types of strains, the key to obtaining good strains is the choice of culture medium or culture medium. Only a suitable culture medium can obtain robust and vigorous fine strains. This experiment started from three aspects: the selection of mother seed culture medium, the selection of original seed culture medium and the selection of cultivated culture material, in order to obtain excellent Phellinus igniarius strains and provide prerequisite guarantee for the successful cultivation of Phellinus igniarius fruiting bodies.

[0066] 1 Selection of the original culture med...

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Abstract

The invention relates to cultivation of a fungus plant, and relates to a culture medium optimizing and short cut log cultivating method of phellinus igniarius. The phellinus igniarius stock culture medium comprises 20g of glucose, 2g of soy peptone, 18g of agar, 0.46g of monopotassium phosphate, 1g of dipotassium phosphate, 0.5g of magnesium sulfate and 1000ml of water. The short cut log cultivating method of phellinus igniarius comprises the following steps of (1) separating phellinus igniarius stock species by using a substantive tissue separation method, (2) culturing phellinus igniarius stock species, (3) culturing phellinus igniarius cultivated species, and (4) preparing short cut log cultivation material. Chinese oak logs are selected as bacteria sticks, so that the cost of artificially cultivating phellinus igniarius is low, the yield is high, the quality is good, the content of effective elements is high and abundant economic returns can be obtained.

Description

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Claims

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Application Information

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Owner 通化长白山药谷集团有限公司
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