New method for building inbred lines of dioecious bivalve molluscs on large scale

Abstract

The invention discloses a new method for building inbred lines of dioecious bivalve molluscs on a large scale. The new method comprises the following steps of acquiring sperms of a monosome; freezing the sperms at ultralow temperature; collecting eggs of a female monosome converted from a male monosome by utilizing a sex reversal characteristic of molluscs on time; activating the frozen sperms, fertilizing with the eggs of the same monosome and incubating to obtain offspring of the same monosome, namely successfully building an inbred line. Different from the traditional habit of building an inbred line by screening spatial hermaphroditic monosomes from dioecious molluscs, the new method has the advantages that the inbred lines of various dioecious bivalve molluscs are built on a large scale, the gene purification speed of the dioecious molluscs is improved, a variety breeding cycle is shortened, and a solid foundation is laid for building pure lines (new varieties); no heterologous gene is imported, and the new method has the advantages of simple operation, high practicability, convenience for promotion and the like.

Description

Technical field: [0001] The invention belongs to the technical field of shellfish seed breeding in marine agriculture, and specifically relates to a new method for large-scale construction of dioecious bivalve inbred lines. Background technique: [0002] Most bivalve molluscs are dioecious, such as clams, cockles, clams, mussels, oysters, etc.; a few species are hermaphrodite, such as bay scallops. Under normal circumstances, an individual of the hermaphrodite shellfish will only produce a single sex. After the gonads mature, an individual can only produce one kind of gametes, namely eggs or sperm; each individual of the hermaphrodite shellfish will produce two at the same time during the development of the gonads. Types of gametes, ie eggs and sperm. [0003] Shellfish often undergo sexual reversal. The so-called sexual reversal refers to the fact that there are both male and female reproductive organs in the shellfish, but they generally exhibit one kind, and sometimes, the othe...

AI Technical Summary

Problems solved by technology

Although the comparison file can construct inbred lines, in most cases, the proportion of hermaphrodites in dioecious shellfish is relatively low, and some special species are even difficult to occur, so the construction of inbred lines still faces many difficulties

In particular, most of the hermaphroditic individuals screened from dioecious shellfish have a large number of sperm and a very small number of eggs, a high rate of embryo deformity during fertilization, and too few inbred offspring. difficult to survive

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