Efficient chrysanthemum cross-breeding method

A hybrid breeding and high-efficiency technology, applied in the fields of botanical equipment and methods, applications, and plant genetic improvement, can solve the problems of great differences in seed setting rate, prolonged breeding cycle, low seed setting rate, etc. Shorter breeding years, accurate and reliable results

Active Publication Date: 2014-06-04
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, due to the great difference in the seed setting rate of different varieties, some varieties, especially those with high petals such as honeycomb type and rosette type, have low seed setting rate, few seeds, and low plumpness. It is also relatively low, and it is difficult to obtain a large number of seedling offspring, which greatly affects the breeding efficiency
In addition, the seedlings grow slowly after sowing in the field, and it is difficult to obtain a large group of strains in that year. After the primary selection in the year, re-selection and optimization in the second and third years are required, which also prolongs the breeding cycle.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0037] 1) Preservation and disinfection of seeds

[0038] The chrysanthemum hybrid seeds were harvested, dried in the shade, placed in kraft paper bags, and stored in a refrigerator at 4°C.

[0039] After the seeds were washed with tap water for 15 minutes, they were soaked in sterile water for 24 hours. Then disinfect with 75% ethanol by volume for 1 min on the ultra-clean workbench, and then disinfect with 5% sodium hypochlorite for 50 min. Wherein, the sodium hypochlorite solution needs to add the surfactant TritonX-100 of 0.3% by volume of the sodium hypochlorite solution. Finally, rinse with sterile water 3 times, each rinse time is about 2min.

[0040] 2) Aseptic seeding

[0041] The sterilized seeds were inoculated in a petri dish, and the dish was filled with 15g L -1 Sucrose-based MS medium, inoculated with 20 seeds per dish. After 15 days, the germinated seeds were transferred to MS medium.

[0042] 3) Obtaining hybrid offspring lines

[0043] After culturing ...

Embodiment 2

[0046] The seeds of the potted chrysanthemum cultivars 'Zhongshan Zaobai' and 'Jinling Sunshine' were harvested and dried in the shade, placed in kraft paper bags, and stored in a refrigerator at 4°C.

[0047] When disinfecting, the seeds are first rinsed with tap water for 15 minutes, and then soaked in sterile water for 24 hours. Then disinfect with 75% ethanol by volume for 1 min on the ultra-clean workbench, and then disinfect with 5% sodium hypochlorite for 50 min. Wherein, the sodium hypochlorite solution needs to add the surfactant TritonX-100 of 0.3% by volume of the sodium hypochlorite solution. Finally, rinse with sterile water 3 times, each rinse time is about 2min.

[0048] The sterilized seeds were inoculated in a petri dish, and the dish was filled with 15g·L -1 Sucrose-based MS medium, inoculated with 20 seeds per dish. After 15 days, the germinated seeds were transferred to MS medium.

[0049] Taking conventional sowing as a control, sow the shade-dried see...

Embodiment 3

[0054] In November 2012, the cultivated chrysanthemum variety 'Feeling green dark' was used as the female parent and 'Mary' was used as the male parent for conventional artificial hybridization. Due to the low seed setting rate of the female parent, and the honeycomb type of the male parent, high petal sex and less pollen, resulting in The seed setting rate of the hybrid offspring was extremely low, and only 18 hybrid seeds were obtained from 428 inflorescences artificially pollinated, of which 12 were full seeds, and the other 6 were not fully mature and full. In order to ensure the germination rate and seedling rate of the seeds, and to obtain the strain population of hybrid offspring in the same year, the steps of "preservation and disinfection of seeds" and "sterile sowing" described in Example 1 of the present invention were taken, and 18 seeds All germinate successfully, and the germination rate is 100%; then carry out the step of "obtaining the hybrid progeny strain" des...

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Abstract

The invention discloses an efficient chrysanthemum cross-breeding method, and belongs to the field of biotechnological breeding. The method comprises the following steps: sterilizing hybrid seeds by 75 percent ethanol for 1 minute, sterilizing the hybrid seeds by 5 percent sodium hypochlorite for 50 minutes, sowing the seeds on a 15g.L<-1> sucrose-containing MS culture medium, and performing further expanding propagation and subculture to obtain a great number of aseptic seedlings by combining a tissue culture technology. According to the method, the germination rate and seedling rate of the hybrid seeds are reliably increased; moreover, for a part of chrysanthemum varieties with cross incompatibility, aseptic seedlings obtained by a small number of hybrid seeds can be subcultured and propagated in the current year to obtain a large group of strains, so that the breeding cycle is shortened, and chrysanthemum breeding methods are further enriched.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding, and in particular relates to a method for high-efficiency chrysanthemum cross-breeding. Background technique [0002] Chrysanthemum is one of the top ten traditional famous flowers native to my country and one of the most important flowers in the world. It has been cultivated for more than 1,600 years. . During the Tang Dynasty, Chinese chrysanthemum was introduced to Japan via North Korea. Since the middle of the 17th century, Chinese chrysanthemum was introduced to Holland, France, Britain and other countries, and has become one of the four major cut flowers in the world. At present, there are more than 4,000 chrysanthemum varieties selected and bred in my country, and the total number of varieties in the world is more than 20,000. It is one of the flowers with the highest commodity output value in the world. Chrysanthemum not only plays a very important role in cut flowers and potted flo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 房伟民金诗媛陈发棣管志勇陈素梅蒋甲福滕年军方庚
Owner NANJING AGRICULTURAL UNIVERSITY
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