A kind of method for rapid propagation of hosta mosaic

A technique for flower and leaf hosta and explants, which is applied to the field of rapid propagation of flower and leaf hosta, can solve problems such as affecting factory production and not publicizing operating conditions, and achieves the effects of maintaining genetic stability, short cycle and promoting bud differentiation.

Inactive Publication Date: 2016-05-04
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above-mentioned prior art discloses medium formulations at different stages, it does not disclose specific operating conditions, and the inventor operates according to the above-mentioned formulations for industrial seedlings to reproduce in large quantities, especially in multiple subcultures. During the multiplication process, it was found that with the increase of the number of subcultures, the mutation rate increased rapidly, which seriously affected the factory production. Therefore, the inventors made targeted improvements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The method of rapid propagation of hosta mosaic includes:

[0023] (1) Selection and disinfection of explants: in March, choose sunny weather and directly take the sprouts on the tubers in the field as explants, cut off the buds on the tubers with a knife, and then soak them in a bath filled with saturated soap. soak in a beaker of water for 5 minutes, then rinse with tap water until there is basically no foam, and then repeat the above steps of soaking and rinsing once again, shortening the soaking time to 2 minutes, and the rinsing time with tap water should not be too long to avoid secondary pollution of the explants. For the ultra-clean workbench, soak it in 70% alcohol for 1 minute, wash it twice in sterile water, disinfect it with 5% sodium hypochlorite solution containing two drops of Tween 80 for 10 minutes, and wash it 5 times in sterile water; Disinfect with 0.1% mercuric chloride containing two drops of Tween 80 for 10 minutes, rinse with sterile water 6 time...

Embodiment 2

[0029] The method of rapid propagation of hosta mosaic includes:

[0030] (1) Selection and disinfection of explants: In April, choose sunny weather and directly take the sprouts on the tubers in the field as explants, cut off the buds on the tubers with a knife, and then soak them in a bath filled with saturated soap. in a beaker of water for 7 minutes, then rinse with tap water until there is basically no foam, and then repeat the steps of soaking and rinsing once again, shortening the soaking time to 3 minutes, and the rinsing time with tap water should not be too long to avoid secondary pollution of the explants. For the ultra-clean workbench, soak it in 70% alcohol for 1 minute, rinse it with sterile water twice, disinfect it with 5% sodium hypochlorite solution containing two drops of Tween 80 for 12 minutes, and rinse it with sterile water 5 times; Add 0.1% mercuric chloride containing two drops of Tween 80 to disinfect for 12 minutes, rinse with sterile water 6 times; ...

Embodiment 3

[0036] A method for rapid propagation of hosta mosaic,

[0037] (1) Selection and disinfection of explants: If explants are taken at the beginning of August, the hosta will be dug out in the ground at the beginning of July and washed, and then planted in pots with clean perlite as the matrix, and then placed in Only water in a clean greenhouse; use a knife to cut off the buds on the tubers, and then soak them in a beaker filled with saturated soapy water for 10 minutes, then wash them in tap water until there is almost no foam, and then repeat the above steps of soaking and washing Once, the soaking time was shortened to 4 minutes, and the tap water washing time should not be too long to avoid secondary pollution of the explants. Then, it was transferred to the ultra-clean workbench, soaked in 70% alcohol for 1 minute in the ultra-clean workbench, and washed in sterile water for 2 minutes. Disinfect with 5% sodium hypochlorite solution containing two drops of Tween 80 for 15 m...

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Abstract

The invention relates to a rapid hosta plantagineu propagation method which mainly comprises the following steps: selecting and sterilizing an explant, performing subculture multiplication culture, performing rooting culture and acclimatizing and transplanting. Particularly, in the subculture process, the light intensity is improved, the content of inorganic salts in a culture medium is reduced, and monopotassium phosphate and lactoalbumin hydrolysate are increased. Meanwhile, the technical means of converting a subculture culture medium is adopted, and thus excellent effects of high multiplication speed, stable inheritable characters and high propagation coefficient are achieved.

Description

technical field [0001] The invention relates to a method for propagating hosta, in particular to a rapid propagation method for mosaic hosta. Background technique [0002] Hosta mosaic (Hostaplantagineu) It is a plant of the genus Hosta in the family Liliaceae, a perennial root flower, and a variant of the famous foliage plant Hosta. It has strong complementarity to light-loving flowers and is an excellent variety for landscaping. The introduction and popularization of flower-leaf hosta has greatly improved its ornamental effect and enriched the landscape color of landscaping. The plant of this variety is short and shade-loving, with complex-colored leaves with folds, golden edges and silver hearts intertwined, and the colorful leaves present a magical cluster of flowers. It can be planted in places where the growth of sun-loving flowers is unfavorable, such as the back side of urban high-rise buildings, under the overpass, the shade of the big tree in the courtyard, and t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张金政孙国峰李晓东吴东启林秦文
Owner INST OF BOTANY CHINESE ACAD OF SCI
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