Preparation method of inoculum agent and method and structure of in situ stacking and decomposing of whole corn stalks
A technology for corn stalks and decomposing inoculants is applied in the field of organic fertilizer and its preparation, which can solve the problems of unsuitability for returning corn stalks to the field through mechanical crushing, and achieves the effects of simple method, solving technical difficulties and improving quality.
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Embodiment 1
[0048] Embodiment 1, the first choice of the present application relates to the preparation method of decomposing bacteria agent, which comprises the following steps
[0049] A. Strain screening
[0050] Take 1000g of fresh livestock feces, add 4000mL sterile water, stir for 5min, centrifuge for 10min, and take the supernatant for later use;
[0051] B. Preparation of decomposing bacteria agent
[0052] (1) Preparation of the original strain: Take 1000g of crushed filter paper, 80g of Na2HPO4, 50g of KH2PO4, 75g of peptone and 30L of water with a pH of 6.5~7.5 in a fermenter, sterilize at 121°C for 30min, cool to 55°C±0.5°C, add A The supernatant in the step is 3500mL, kept at 55°C±0.5°C, stirred and cultured at 60 rpm for 120 hours, and the original seed of the fermentation broth is obtained;
[0053] (2) Preparation of mother seed: Take rice bran, crop straw crushed to less than 3mm, water, urea and fermentation liquid original seed, mix evenly according to the mass ratio ...
Embodiment 2
[0057] Embodiment 2, the first choice of the present application relates to the preparation method of decomposing bacteria agent, which comprises the following steps
[0058] A. Strain screening
[0059] Take 1000g of fresh livestock feces, add 4000mL sterile water, stir for 5min, centrifuge for 10min, and take the supernatant for later use;
[0060] B. Preparation of decomposing bacteria agent
[0061] (1) Preparation of the original strain: Take 1000g of crushed filter paper, 80g of Na2HPO4, 50g of KH2PO4, 75g of peptone and 30L of water with a pH of 6.5~7.5 in a fermenter, sterilize at 121°C for 30min, cool to 55°C±0.5°C, add A The supernatant in the step is 3500mL, kept at 55°C±0.5°C, stirred and cultured at 60 rpm for 120 hours, and the original seed of the fermentation broth is obtained;
[0062] (2) Preparation of mother seed: Take rice bran, crop straw crushed to less than 3mm, water, urea and fermentation liquid original seed, mix evenly according to the mass ratio ...
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