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Detection tool for screening susceptible population of bird influenza and human influenza viruses

A technology for human influenza virus and susceptible populations, which is applied in the field of screening avian influenza and human influenza virus susceptible populations to achieve the effects of highly targeted testing and low production costs

Active Publication Date: 2014-06-25
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] However, why when the flu broke out, the people who were infected were mostly the elderly? The root cause has not yet been clearly explained

Method used

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  • Detection tool for screening susceptible population of bird influenza and human influenza viruses
  • Detection tool for screening susceptible population of bird influenza and human influenza viruses
  • Detection tool for screening susceptible population of bird influenza and human influenza viruses

Examples

Experimental program
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Effect test

Embodiment Construction

[0040] Whole saliva sample collection: male patients aged 45-65 with hepatitis B, 17 cases of liver cirrhosis and patients with liver cancer; 26 cases of male and female patients with type 2 diabetes aged 50-65; men with gastric cancer aged 50-65 27 patients and 30 healthy males and females aged 45-65 years old, two hours after meals, between 9:00 and 10:00, rinsed with normal saline for three times and quickly collected the whole saliva secreted naturally. At least 1 mL of saliva was collected and immediately placed on ice, and protease inhibitors (10 μL per mL of saliva) were added to prevent protein degradation.

[0041] The main experimental steps are:

[0042] 1) Preparation of lectin chips;

[0043] 1.1) Take the epoxy film base for use;

[0044] 1.2) Prepare the lectin into a sample solution with a concentration of 1mg / mL;

[0045] 1.3) Add the prepared spotting solution into the 384-well plate; then use the crystal core 48 spotting system to spot the sample on the e...

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Abstract

The invention provides a special detection tool capable of screening susceptible population of bird influenza and human influenza viruses. The detection tool is a lectin testing chip aiming at a saliva sample and adopts an epoxidation film base; two types of lectins including MAL-II (Maackia Amurensis Leukoagglutinin-II) and SNA (Sambucus Nigra Agglutinin) are fixed on the epoxidation film base by sample application. A corresponding lectin chip kit comprises the lectin testing chip, confining liquid, washing liquid and an incubation buffer solution. The invention further optimizes a preparation and treatment method of the lectin testing chip so as to establish an optimal reaction system for detecting saliva glycoprotein carbohydrate chain end sialic acid alpha2-3 and alpha2-6 carbohydrate chain structures in the saliva sample. The product provided by the invention is low in production cost and strong in testing pertinence; the inherent advantages of the lectin chip are combined to rapidly, simply and efficiently detect information of an evaluating result of the susceptible population of the bird influenza and human influenza viruses without damages.

Description

technical field [0001] The invention relates to a method for screening avian influenza and human influenza virus susceptible populations and a special detection tool. Background technique [0002] Lectins are non-immune-derived, non-enzymatically active sugar-binding proteins that can specifically recognize and bind to a specific sequence of sugar chains in monosaccharides or polysaccharides with a special structure. Up to now, more than 100 plant-derived lectins have been found in nature. Lectins have various applications: such as the diagnosis of malignant tumors and other diseases, the design of antibacterial and antiviral drugs, the design of "biological missiles", and the identification and analysis of sugar chain structures, etc. With the introduction of microarray technology into the field of glycomics research, lectins have attracted more and more attention as an important tool for decoding the structure of sugar chains. According to the specific interaction betwee...

Claims

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Application Information

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IPC IPC(8): G01N33/53
CPCG01N33/56983G01N2333/11G01N2333/4724
Inventor 李铮秦棪楠钟耀刚于汉杰鱼静敏陈琳朱光
Owner NORTHWEST UNIV
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