Cell-activating essence product as well as preparation method and application thereof

A technology of living cells and essence, applied in the field of cell biology, can solve the problems of long time, rejection, short survival time, etc., to increase the content, avoid allergies, and increase the effect of curative effect.

Active Publication Date: 2014-07-02
南京华方生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Transplant stem cells for wound repair. The probability of stem cells differentiation into tissue cells is very low and the survival time after transplantation is very short, so they cannot effectively function; plant or recombinant factor preparatio

Method used

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  • Cell-activating essence product as well as preparation method and application thereof
  • Cell-activating essence product as well as preparation method and application thereof
  • Cell-activating essence product as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0054] Example 1

[0055] A conditioned medium comprising the following components in parts by weight: 0.05 parts of basic fibroblast growth factor, 0.05 parts of epidermal growth factor, 95 parts of DMEM / F12, 1 part of antibiotics, 1 part of glutamate, non-essential amino acids 1 part, 1 part antioxidant.

Example Embodiment

[0056] Example 2

[0057] A conditioned medium includes the following components in parts by weight: 0.05 parts of epidermal growth factor, 95.9 parts of DMEM / F12, 1 part of antibiotics, 1 part of glutamate, 1 part of non-essential amino acids, and 1 part of antioxidants.

[0058]

Example Embodiment

[0059] Example 3

[0060] A conditioned medium includes the following components in parts by weight: 0.05 parts of basic fibroblast growth factor, 94.9 parts of DMEM / F12, 1 part of antibiotics, 1 part of glutamate, and 1 part of non-essential amino acids.

[0061]

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Abstract

The invention discloses a conditioned medium which comprises the following components in parts by weight: 0-0.05 part of basic fibroblast growth factor, 0-0.05 part of epidermal growth factor, 94.9-95.9 parts of dulbecco modified eagle medium (DMEM)/ F12, 1 part of antibiotics, 1 part of glutamate, 1 part of non-essential amino acid and 0-1 part of antioxidant. The invention also discloses a cell-activating essence product and a preparation method of the cell-activating essence product. The invention further discloses the application of the cell-activating essence product. The used conditioned medium does not contain animal-based substances, so that the problems of irritability, infection and the like caused by the differences among species can be avoided. Adipose-derived stem cells (ADSCs) are separated by stages by virtue of the mixed enzyme; and the yield of the ADSCs is 3000-5000/ mg. The conditioned medium is used for promoting secretion, so that the content of protein active factor can be increased and the treatment effect of the cell-activating essence product can be improved.

Description

technical field [0001] The invention belongs to the field of cell biology, and in particular relates to a live cell essence product and its preparation method and application. Live Cell Essence is a high-tech product with active substances derived from human adipose stem cells (Adipose Stem Cell Paracrine Protein Factors (APP) for short), which can be widely used in cosmetic fields such as beauty and anti-aging and medical fields such as burns. Background technique [0002] At present, the products related to the application of stem cells in beauty at home and abroad are mainly concentrated in the following countries: Japan's human placenta (Lai Naikang), placenta (Messman); Switzerland's sheep placenta (LFS); South Korea's fat stem cell protein (AAPE), etc. [0003] Lai Naikang is a placenta injection produced by Japan's JBP advanced biotechnology. It is one of the earliest products approved by the Ministry of Health and Welfare. Mesman was successfully developed by Japan ...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A61K35/12A61K8/98A61P17/00A61Q19/00A61Q19/08
Inventor 陈礼明曹振芳杨桂娟
Owner 南京华方生物工程有限公司
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