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Method for obtaining hemopoietic stem cell by using three-dimensional induction system

A technology of hematopoietic stem cells and three-dimensional cells, applied in the field of efficiently obtaining hematopoietic stem cells, can solve the problem of not being able to obtain myeloid and lymphoid cells at the same time

Active Publication Date: 2014-07-23
ZHEJIANG UNIV
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  • Application Information

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Problems solved by technology

Functionally, although these cells can form part of hematopoietic colonies, generate erythroid progenitor cells, granulocyte progenitor cells, macrophages, etc., but cannot obtain myeloid and lymphoid cells at the same time

Method used

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  • Method for obtaining hemopoietic stem cell by using three-dimensional induction system
  • Method for obtaining hemopoietic stem cell by using three-dimensional induction system
  • Method for obtaining hemopoietic stem cell by using three-dimensional induction system

Examples

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Embodiment 1

[0031] Example 1 Preparation of three-dimensional induction system to induce differentiation of pluripotent stem cells into blood cells.

[0032] The present invention provides three-dimensional cell culture materials using three-dimensional cell culture materials, including hydrogel, seaweed, nanomaterials, etc., and / or combined bone marrow stromal cells, and / or combined mouse stromal cell lines OP9 or OP9-DL1 , and / or combine multiple mesoderm-inducing factors and hematopoietic-related factors to induce pluripotent stem cells to differentiate into hematopoietic stem cells. See the specific plan figure 1 and figure 2 .

[0033] 1. The materials and reagents used in the examples can be obtained from commercial sources unless otherwise specified.

[0034] 2. Culture, expansion and passage of pluripotent stem cells

[0035] ①Preparation of trophoblast MEF (purchased from Shanghai Stance Biological Technology Co., Ltd.): according to the conventional method.

[0036] ②Re...

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Abstract

The invention provides a method for obtaining a hemopoietic stem cell by using a three-dimensional induction system. A three-dimensional cell culture medium or cell culture bracket, such as a three-dimensional cell culture system made of the materials such as hydrogel, seaweed and the like is utilized, and / or combined with matrix cells such as bone marrow cell, mouse bone marrow cell lines OP9, OP9DL1 and the like, and / or combined with a plurality of factors including mesoderm induction factors, hematopoietic growth factor and the like to induce multipotent stem cells to differentiate into hemopoietic stem cells. A new method for obtaining the hemopoietic stem cells is built. The system for efficiently inducing the multipotent stem cells to differentiate into the hemopoietic stem cells by using a three-dimensional induction system and / or combining with matrix cells such as bone marrow cell and the like and / or a plurality of factors is built for the first time. Theoretical basis and a technology platform are provided for obtaining clinically available hemopoietic stem cells, and a new method and a new concept are developed for application of hematopoietic cells derived from multipotent stem cells in the fields such as disease mechanism exploration, drug screening and the like.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method for efficiently obtaining hematopoietic stem cells by using a three-dimensional induction system. Background technique [0002] The occurrence and development of hematopoiesis is a complex process. It begins at the end of the second week of human embryos. At this time, a group of undifferentiated, self-renewing extraembryonic mesoderm cells located on the wall of the yolk sac form the blood island, which becomes the initial human hematopoietic center. From the 6th week of the embryo, the hematopoietic stem cells (Hematopoietic Stem Cells, HSCs) produced by the blood island of the yolk sac migrate to the liver along with the blood flow, and implant in the liver, starting the hematopoietic period of the liver. In the third month of the embryo, the long bone marrow has begun to produce blood. At the 8th month, bone marrow hematopoiesis has been highly developed. At thi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0789
CPCC12N5/0647C12N2501/02C12N2501/125C12N2501/14C12N2501/145C12N2501/155C12N2501/165C12N2501/22C12N2501/2302C12N2501/2303C12N2501/2306C12N2501/2307C12N2501/26C12N2506/02C12N2513/00
Inventor 黄河徐玉林于晓红
Owner ZHEJIANG UNIV
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