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A biocontrol bacterial fertilizer for preventing and controlling crop soil-borne diseases and its production process and application

A technology of soil-borne diseases and bio-control bacteria, which is applied in the fields of application, plant growth regulators, botanical equipment and methods, etc. It can solve the problems of unsatisfactory control effects, few types of chemical fungicides, and environmental pollution of chemical agents. , to achieve low cost, improve soil fertility, and easy access

Active Publication Date: 2015-12-02
广州绿创宝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, the use of plants with disease-resistant strains can achieve cost-effective control effects, but it is not easy to obtain an ideal disease-resistant strain, and resistant varieties often appear resistant to disease after a few years of promotion. on the other hand, chemical control plays a very important role in the control of soil-borne diseases of crops due to its advantages of high control efficiency, fast speed, and wide antibacterial spectrum. Limitations of two factors: first, there are few types of high-efficiency and low-toxic chemical fungicides, and most of them are still highly toxic, so it is not a long-term solution to kill bacteria with poison; second, the impact of environmental factors on the control effect is not small , when the environmental conditions are suitable for the invasion and spread of pathogenic bacteria, the control effect is often unsatisfactory; the third is that chemical agents are likely to cause environmental pollution, endangering human daily life

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 different bacterial strains are to the inhibition test research of Botrytis cinerea

[0019] Preparation of PDA medium: Weigh 200g of peeled potatoes, cut into pieces and boil for 30min, then filter the juice with gauze, add 20.0g of glucose and 20.0g of agar, add water to 1000mL after dissolving, and divide into 10 triangles of 150mL bottle, sterilized at 121°C for 30 minutes.

[0020] Under sterile conditions, take 20 mL of PDA medium that has been sterilized and cooled to about 45°C, and add it to a petri dish to make a PDA plate. After standing for 24 hours, inoculate the mycelium of the pathogen Botrytis cinerea in the center of the plate, and culture at 22°C for 4 days. In the Botrytis cinerea plate that has grown, use a diameter 1cm puncher to cut out colony circles at the edge of the colony for subsequent use.

[0021] Take the Bacillus cereus fermented liquid and the Pseudomonas chloropinus fermented liquid prepared according to the process of ...

Embodiment 2

[0027] The preparation of embodiment 2 biocontrol bacteria fertilizer

[0028] (1) Preparation of liquid seed medium: Weigh 10g of peptone, 5g of beef extract, and 5g of NaCl, add purified water, stir and mix well, make up water to 1000mL, adjust pH=7.0, divide into ten 150mL Erlenmeyer flasks, 121°C Sterilize for 30 minutes.

[0029] (2) Preparation of liquid fermentation medium: Weigh 1kg of peptone, 0.5kg of beef extract, and 0.5kg of NaCl, add purified water and mix well, make up water to 100L, adjust the pH to 7.0, and sterilize at 121°C for 30 minutes.

[0030] (3) Insert Bacillus cereus into the liquid medium in the above-mentioned Erlenmeyer flask, shake and culture at 30°C and 180r / min for 30 hours to form a seed liquid; inoculate the seed liquid into the fermentation medium at a volume ratio of 1:500 Fermentation production is carried out in the liquid medium in the tank. The basic working parameters of the fermenter are: temperature 30°C, stirring speed 200r / min,...

Embodiment 3

[0034] Example 3 Experimental Research of Biocontrol Bacterial Fertilizer on Controlling Botrytis cinerea

[0035] Two tomato planting fields with consistent soil properties, uniform and medium fertility, and heavy gray mold incidence were selected and divided into experimental fields and control fields, and the tomatoes planted in these two fields were treated as follows:

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PUM

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Abstract

The invention discloses a biological antibacterial fertilizer for preventing and controlling soil-borne diseases of crops as well as a production process and an application of the biological antibacterial fertilizer. The production process comprises the steps of culturing to obtain bacillus cereus and pseudomonas choloeaphtis fermentation liquids, and inoculating the two fermentation liquids into cured livestock manure and straw according to a certain proportion to compost and ferment to form the biological antibacterial fertilizer for preventing and controlling soil-borne diseases of crops. The biological antibacterial fertilizer disclosed by the invention can be used for preventing and controlling vegetable botrytis cinerea and increasing the yield of vegetables, safe, pollution-free and easy to realize large-scale production.

Description

technical field [0001] The invention belongs to the technical field of microbial organic fertilizers, and in particular relates to a biocontrol bacterial fertilizer for preventing and controlling soil-borne diseases of crops and its production process and application. Background technique [0002] Soil-borne diseases of crops cause huge economic losses to agricultural production every year, so the prevention and control of diseases has become the top priority of current agricultural science. At present, the prevention and control methods of soil-borne diseases of crops are mainly based on the use of disease-resistant strains of plants and chemical control. On the one hand, the use of plants with disease-resistant strains can achieve cost-effective control effects, but it is not easy to obtain an ideal disease-resistant strain, and resistant varieties often appear resistant to disease after a few years of promotion. on the other hand, chemical control plays a very important ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F17/00C05F3/00C05F11/08A01N63/02A01P3/00
CPCY02W30/43Y02A40/20Y02W30/40
Inventor 段占娥
Owner 广州绿创宝生物科技有限公司
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