A method for tissue culture and rapid propagation of Atractylodes atractylodes
A technology of tissue culture rapid propagation and herb atractylodes, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of easy degradation of quality, a large number of rhizome materials, and low reproductive coefficient of seeds, and achieve the effect of alleviating the contradiction between supply and demand
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Embodiment 1
[0024] Embodiment 1: a kind of method for tissue culture and rapid propagation of Atractylodes atractylodes, comprising the following steps:
[0025] 1. Seed treatment and disinfection
[0026] Put a filter paper of appropriate size in the petri dish, and select the large and plump wild Atractylodes atractylodis harvested in autumn ( Atractylodeslancea (Thunb.)DC.) seeds, washed with washing powder, and then soaked in appropriate amount of water (just below the seeds) for 0.5h; after soaking, rinsed repeatedly with running water, and then rinsed twice with distilled water; The seeds were soaked in 70% ethanol by volume for 30 seconds, then soaked in 0.1% mercuric chloride solution by mass for 15 minutes, rinsed three times with sterile water, and dried on sterile filter paper for later use.
[0027] 2. Establishment of sterile culture system
[0028] Put the above-mentioned sterilized seeds into the germination medium, 1 grain per bottle; germinate after 14 days, and grow in...
Embodiment 2
[0041] Embodiment 2: basically the same as Embodiment 1, the difference is:
[0042] The set culture conditions involved in steps 2 to 6 are as follows: light intensity: 2500lx; light time: 15h / d; culture temperature: 24°C.
[0043]The disinfection and sterilization treatment method in step (1) is: soak the cleaned seeds in clean water for 1 hour. After soaking, rinse them repeatedly with running water, then rinse them twice with distilled water, and then soak them in an ethanol solution with a volume percentage of 70%. 20s, then soaked in 0.1% mercuric chloride solution for 18 minutes, and finally rinsed with sterile water 5 times, blotted dry on sterile filter paper for later use.
[0044] In step (2), germinate under the set culture conditions for 10 days, and the pH of the germination medium is 6.2.
[0045] The callus induction and differentiation medium in step (3) is: MS+0.5mg / L 6-benzylaminoadenine+0.05mg / L naphthaleneacetic acid+3% sucrose+mass percent 0.75% Agar, p...
Embodiment 3
[0049] Embodiment 3: basically the same as Embodiment 1, the difference is:
[0050] The set culture conditions involved in steps 2 to 6 are as follows: light intensity: 2500lx; light time: 13h / d; culture temperature: 22°C.
[0051] The disinfection and sterilization treatment method in step (1) is: soak the cleaned seeds in clean water for 0.7h, after soaking, rinse repeatedly with running water, then rinse twice with distilled water, and then soak in 70% ethanol solution by volume For about 25 seconds, soak in 0.1% mercuric chloride solution for 17 minutes, rinse with sterile water for 5 times, and blot dry on sterile filter paper for later use.
[0052] In step (2), germinate under the set culture conditions for 13 days, and the pH of the germination medium is 6.0.
[0053] The callus induction and differentiation medium in step (3) is: MS+1.5mg / L 6-benzylaminoadenine+0.15mg / L naphthalene acetic acid+mass percentage of 3% sucrose+mass percentage of 0.75% Agar, pH 6.0.
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