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Method for detecting concentration of trace immunoglobulin G in human serum

A technology of human immunoglobulin and immunoglobulin, which is applied in the direction of measuring devices, biological testing, material inspection products, etc., to achieve the effect of improving sensitivity and selectivity, and detecting accurately, conveniently and quickly

Inactive Publication Date: 2014-08-06
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Abstract
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Problems solved by technology

At present, the application of fluorescein isothiocyanate and nano-gold energy transfer method in the detection of immunoglobulin G has not been reported at home and abroad

Method used

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  • Method for detecting concentration of trace immunoglobulin G in human serum
  • Method for detecting concentration of trace immunoglobulin G in human serum

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Experimental program
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Embodiment Construction

[0023] (1) Preparation of nano gold solution:

[0024] Add 5 milliliters of 5 mmol / L chloroauric acid into 100 milliliters of sub-boiling water, heat to boiling under constant stirring, then quickly add 5 milliliters of citric acid solution with a mass volume concentration of 0.1%, keep boiling for 10 minutes, and the solution The color gradually turns into wine red, stop heating, and centrifuge at 10,000 rpm for 10 minutes to remove excess citric acid to obtain a nano-gold solution with a concentration of 50 nanomoles / liter, and store it at 4°C. spare.

[0025] (2) Preparation of goat anti-human immunoglobulin G antibody-labeled fluorescein isothiocyanate solution:

[0026] 20 ml of 1.0 x 10 -5 mol / L fluorescein isothiocyanate, 100 μl of 20 mg / ml 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 100 μl of 10 mg / ml N-hydroxysuccinimide was mixed and stirred at room temperature for 0.5 hours, then 100 μl of 1 mg / ml goat anti-human immunoglobulin G antibody was ...

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Abstract

The invention discloses a method for detecting the concentration of trace immunoglobulin G in human serum. An energy transferring system with stable performance consists of goat anti human immunoglobulin G antibody-labeled fluorescein isothiocyanate serving as an energy transferring supplier and nano gold serving as a receptor; the goat anti human immunoglobulin G antibody-labeled fluorescein isothiocyanate transmits energy to the nano gold, so that fluorescence of the fluorescein isothiocyanate is quenched; however, after immunoglobulin G is added, the fluorescence of the fluorescein isothiocyanate is recovered; furthermore, the fluorescence recovery value and the concentration of the immunoglobulin G are in good linear relation within a range of 4.0-220.0 nanograms per milliliter, so that a novel method for detecting the immunoglobulin G is built. According to the method disclosed by the invention, the shortcomings of low sensitivity, complicated operation and the like during detection in the prior art are overcome, and the immunoglobulin G in the human serum can be conveniently and quickly detected.

Description

technical field [0001] The invention relates to a method for rapidly detecting the concentration of trace immunoglobulin G in human serum by using fluorescein isothiocyanate and nano-gold energy transfer technology. Background technique [0002] Immunoglobulin G is the immune protein with the highest content in serum, accounting for about 70% to 80% of serum immune proteins. It is the main component of antibodies formed after the body's second immune response, and plays an important role in the body's defense mechanism , is clinically used as an important indicator for the diagnosis and curative effect evaluation of various diseases, so its qualitative and quantitative detection is very important. At present, the main methods for detecting human immunoglobulin G at home and abroad include enzyme-linked immunoassay, voltage immunosensing technology, electrochemiluminescence immunoassay, fluorescence sensing technology, ultraviolet absorption photometry, etc. Some of the abov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/64
CPCG01N33/6854G01N33/542
Inventor 陶慧林廖秀芬徐铭泽谢襄漓钟福新易忠胜
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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