72 results about "Fluoreszein" patented technology

The invention relates to a polyamine micromolecular compound, comprising the following structures described in the specification, wherein M<x+> is 0, Zn<2+>, Ga<3+>, Gd<3+>, Ca<2+> or other divalent metal ions and trivalent metal ions; S is a reporter group (including a nuclide labeling prothetic group, a paramagnet, a fluorescein or a microvesicle), such as the nuclide labeling prothetic group: -<11>CH3, -CH2CH2<18>F, -CH2CH2(OCH2CH2)2NHCOC6H4<18>F-p or -CH2CH2(OCH2CH2)2NH-CH2CH2<18>F; R is -H, -OCH3, -OCH2CH3 or -Cl; and R1, R2, R3 and R4 are hydrogen, carboxyl, alkane, alkylene or heteroalkyl. The invention further relates to application of the compound in preparing cell death or apoptosis developers of target phosphatidyl serine (PS) and/or apoptotic cell early free Zn<2+>. The compound provided by the invention is a specific multiamine micromolecular developer for target phosphatidyl serine (PS) and/or apoptotic cell early free Zn<2+>, which can be used for monitoring curative effects of PS-related anti-tumor chemotherapy, radiotherapy, biological therapy and the like; the compound can be used for early differential diagnosis of neurodegenerative diseases (senile dementia and Parkinson's disease), cerebral apoplexy, AIDS (Acquired immune deficiency syndrome), thrombus, atheromatous plaque and myocardial infarction; and the compound can also be used for differential diagnosis and curative effect monitoring of other diseases related to PS expression in the cell death or apoptosis process, such as inflammation development and anti-inflammation therapeutic development.

The invention discloses an immunofluorescence dipstick component for quickly and quantitatively detecting protein of a plurality of types and a detection card component prepared from the same and a preparation method thereof, wherein the protein of a plurality of types comprises muscle hemoglobin/creatine kinase isoenzyme/troponin I; the dipstick component comprises a dipstick consisting of a bottom liner, a water-absorbing pad, a coated analysis membrane and a sample pad and independently packaged fluorescein mark specific antibodies; three detection lines and a quality control line are arranged on the coated analysis membrane; the detection line on the coated analysis membrane is respectively coated with an anti-myoglobin monoclonal antibody line, an anti-creatine kinase isozyme monoclonal antibody line and a troponin I monoclonal antibody line; the quality control line is coated with a rabbit IgG antibody; the fluorescein mark specific antibodies comprise the anti-myoglobin monoclonal antibody line, the anti-creatine kinase isozyme monoclonal antibody line, an troponin I monoclonal antibody and an anti-rabbit IgG antibody; and the detection card component comprises the dipstick, a card box consisting of a cover plate and a back plate and independently packaged platinum porphyrin mark specific antibodies and can synchronously detect the muscle hemoglobin/creatine kinase isoenzyme/troponin I, is simple to operate, is quick and sensitive, and has good specificity.

The invention provides a test paper used for detecting acute myocardial infarction, and a preparation method and an application method thereof. The test paper comprises a base plate and sequentially closely connected components adhered to the base plate, wherein the components are a sample absorption pad, a bonding pad, a chromatography membrane, and a water absorption pad. The bonding pad is coated with a glucan-antibody-fluorescein mixed marker. The chromatography membrane is provided with a detection band and a control band. A monoclonal antibody is fixed on the detection band. A rabbit anti-mouse IgG antibody which can be speficically bound with the glucan-antibody-fluorescein mixed marker is fixed on the control band. The sensitivity range of the reagent provided by the invention reaches 0.1ng/L. With the test paper, quantitative detections upon trace myoglobin, troponin I and creatine kinase isoenzyme in myocardial infarction patient serum can be realized. A result can be obtained in 3-5 minutes after sample spotting. The operation is simple, and professional operation is not needed.

The invention relates to the technical field of medical detection, in particular to a detection kit for minute residues of B-cell acute lymphocyte leukemia. The detection kit comprises anti CD10, CD19, CD20, CD34, CD38 and CD45 antibodies labeled by different fluoresceins. The detection kit comprises six monoclonal antibody combinations of leukocyte differentiation antigen, and a fluorescence labeling and flow cytometric detection technology is combined to realize that one-time flow cytometric detection can quickly and accurately identify the level and typing of abnormal cells of the minute residues of the B-cell acute lymphocyte leukemia, the obtained detection index can be used as an intermediate result to intuitively judge the prognosis conditions of B-cell acute lymphocyte leukemia patients, and thus important guiding significance is provided for the formulation of clinical treatment schemes for the leukemia patients.

The invention discloses an immunofluorescence chromatography test strip for detecting a platelet antibody and a detection method of the platelet antibody. The immunofluorescence chromatography test strip comprises a reaction film, a sample pad, an adsorption pad and a bottom plate, wherein the reaction film is placed on the bottom plate; a detection belt and a quality control belt are fixed onto the reaction film; the sample pad is overlapped with part of one side of the reaction film and placed on the reaction film and the bottom plate; the absorption pad is overlapped with part of the other side of the reaction film and placed on the reaction film and bottom plate; a sampling point is arranged on each of two sides of the sample pad; sample and fluorescence labeled secondary antibodies are dropped and washed; and after the reaction is done, the reaction result is indicated through fluorescein isothiocyanate labeled secondary antibodies. Due to the mode, according to the immunofluorescence chromatography test strip for detecting the platelet antibody and the detection method disclosed by the invention, the detection process is simple, efficient, accurate, low in cost and practical; semi-quantitative or quantitative detection can be realized; safe, efficient and scientific platelet transfusion in clinic can be guaranteed; and the precious platelet resources also can be saved.

The invention relates to a nanometer magnetic particle chemiluminescent assay kit for a cancer antigen CA15-3, and a preparation method and a detection method thereof. The kit comprises a solution containing a fluorescein-mark labeled cancer antigen CA15-3 antibody, suspension of fluorescein antibody coated magnetic particles and a solution containing an alkaline phosphatase labeled cancer antigen CA15-3 antibody, wherein the alkaline phosphatase labeled cancer antigen CA15-3 antibody is formed by connecting alkaline phosphatase and the cancer antigen CA15-3 antibody through a crosslinking agent SMCC and 2-IT. According to the invention, the cancer antigen CA15-3 can be quantitatively detected with low cost, high accuracy degree and high precision degree.

The invention belongs to the field of biochemical industry, and particularly relates to a preparation method and application of compounds FL-O-T for detecting tyrosinase. The compounds FL-O-T have a structural general formula shown in the description, wherein R1, R2 and R3 are hydrogen, alkyl or other common substituent groups. The compounds FL-O-T can be used as a fluorescent probe for detectingthe tyrosinase, and the compounds FL-O-T are synthesized by a nucleophilic substitution reaction of a fluorescein derivative and m-hydroxybenzyl bromide. The compounds FL-O-T provided by the inventionare synthesized by the nucleophilic substitution reaction of the fluorescein derivative FT-OH and the m-hydroxybenzyl bromide; and the target compounds have high chemoselectivity, can track and detect the tyrosinase in vitro and in vivo, and successfully monitor activity of the tyrosinase in cancer cells and zebrafish.

Immunoassay reagents, methods and test kits for the specific quantification of vancomycin in a test sample are disclosed. The reagent comprises antibodies prepared with immunogens of FIG. 6 wherein P is an immunogenic carrier material and X is a linking moiety.

Also described is the synthesis of labeled reagents of FIG. 8 wherein Q is a detectable moiety, preferably fluorescein or a fluorescein derivative, and X is a linking moiety.

The invention relates to a porcine deltacoronavirus LFD-RPA rapid detection primer probe set and a kit, and belongs to the field of biotechnology. The primer probe set includes an upstream primer, a downstream primer and a probe; the nucleotide sequence of the upstream primer is shown in SEQ ID NO.1, the downstream primer is a substance of which the nucleotide sequence is shown in SEQ ID NO.2 and the 5' end is modified by biotin, and the probe is a substance of which the nucleotide sequence is shown in SEQ ID NO.3, the 5' end is modified by C3Spacer, the 5' end is modified with fluorescein and the thirtieth base from the 5' end is modified by a tetrahydrofuran residue. By adopting the porcine deltacoronavirus LFD-RPA rapid detection primer probe set, porcine deltacoronaviruses can be rapidly detected; the primer probe set is simple and high in sensitivity and specificity and can improve the effectiveness of a current PDCoV controlling scheme.

A phosphorous compound such as STMP is used as a cross-linking agent while making a starch nanoparticle in an emulsion process. Negative charge of the nanoparticle is reduced or reversed by adding cations and/or cationizing the starch optionally while forming the nanoparticles. Anionic active agents, such as fluoride or fluorescein, are optionally incorporated into the nanoparticle during the formation process. For example, a fluoride salt can also be used, which promotes the crosslinking reaction while also providing fluoride in the nanoparticle. The retention of both calcium and fluoride in the nanoparticle is improved when both salts are used. Alternatively, the nanoparticle may be used without added calcium and/or fluoride. The nanoparticles may be useful for tooth remineralization, the treatment of dentinal hypersensitivity, to treat caries, or as a diagnostic agent to locate carious lesions.

The invention provides a fluorescein marked protein tetramer as well as a preparation method and application thereof. The fluorescein marked protein tetramer is formed by combining fluorescein markedstreptavidin and a biotin marked target protein. The biotin marked target protein is in recombinant expression in an HEK293 cell, one Avi Tag is coexpressed at an N-end or a C-end, and furthermore, under the action of a BirA enzyme, a lysine residue group of Avi Tag is connected with biotin. The biotin marked target protein and the fluorescein marked streptavidin are mixed and reacted to obtain the protein tetramer in direct fluorescence marking. The protein tetramer in direct fluorescence marking, which is provided by the invention, can be applied to detection on positive rates of neutralizing antibodies or CAR-T (chimeric antigen recetor-T) cells of target proteins of stream screening immune checkpoints, and as streptavidin and biotin both play a role of cascade amplification, the detection sensitivity of the protein tetramer is greatly improved when being compared with that of fluorescein marked proteins prepared by using other methods.

The invention relates to the technical field of chemiluminiscence immunoassay, in particular to a homogeneous immunoassay method for quenching acridinium ester chemiluminiscence based on an ortho-position touch effect and acridinium ester chemiluminiscence and use equipment. A detection solution adopted in the analysis method comprises a DNA1-conjugate, a DNA2-conjugate, acridinium ester (AE) labeled DNA3, a graphene oxide (GO) combined antioxidant (AOD) and the like. The method is a homogeneous chemiluminiscence immunoassay method, separation and cleaning steps are not needed, the operation is simple, meanwhile, the turnover time (TAT) of clinical examination specimens is greatly shortened, centrifugal treatment is not needed for blood specimens, whole blood loading can be achieved, and adetection report can be given within 5-10 minutes; the acridinium ester derivative (AE) is different from fluorescein (Cy5 and the like) substances and can realize anti-interference chemiluminescencedetection; and the method not only can be used for detecting macromolecular protein and antibodies, but also can be used for realizing immunodetection of chemical small molecules.

The invention discloses a rapid, simple and convenient deafness gene detection method and a kit. The kit comprises a primer combination for detecting deafness gene mutation, the primer combination is composed of G235WF, G235MF, G235WR, SIV72WF, SIV72WR and SIV72MR, and the sequences of the primer combination are as follows: the 5'tail ends of the G235WF, the G235MF, the G235WR, the SIV72WF, the SIV72WR and the SIV72MR are respectively marked with fluorescein FITC, TAMATA, biotin biotin, biotin biotin, fluorescein FAM and DIG. Experiments prove that the kit disclosed by the invention can be used for rapidly, accurately and sensitively detecting mutation conditions of two sites of GJB2: c.235delC and SLC26A4:c.919-2A>G.

The invention discloses a bioluminescent engineered bacterium composition, and a preparation method and application thereof. The bioluminescent engineered bacterium composition disclosed by the invention comprises bioluminescent engineered bacteria, a luminescent substrate, photosensitizer molecules and biomacromolecules capable of being gelatinized in situ. The invention also discloses application of the bioluminescent engineered bacterium composition in tumour photodynamic therapy and immunotherapy. According to the invention, plasmids expressing firefly luciferase are transfected into attenuated salmonella to construct bioluminescent engineered bacteria; the bacteria can generate yellow-green light with the wavelength of 540-600 nm when being co-incubated with a substrate D-fluorescein,so as to excite Ce6 to generate singlet oxygen to realize photodynamic therapy of tumours; and meanwhile, the engineered bacteria can be used as an immunologic adjuvant to activate anti-tumour immuneresponse of an organism and enhance tumour photodynamic therapy, so that efficient synergistic therapy of tumours is realized.