Porcine deltacoronavirus LFD-RPA rapid detection primer probe set and kit

Abstract

The invention relates to a porcine deltacoronavirus LFD-RPA rapid detection primer probe set and a kit, and belongs to the field of biotechnology. The primer probe set includes an upstream primer, a downstream primer and a probe; the nucleotide sequence of the upstream primer is shown in SEQ ID NO.1, the downstream primer is a substance of which the nucleotide sequence is shown in SEQ ID NO.2 and the 5' end is modified by biotin, and the probe is a substance of which the nucleotide sequence is shown in SEQ ID NO.3, the 5' end is modified by C3Spacer, the 5' end is modified with fluorescein and the thirtieth base from the 5' end is modified by a tetrahydrofuran residue. By adopting the porcine deltacoronavirus LFD-RPA rapid detection primer probe set, porcine deltacoronaviruses can be rapidly detected; the primer probe set is simple and high in sensitivity and specificity and can improve the effectiveness of a current PDCoV controlling scheme.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer probe set and a kit for rapid detection of porcine delta coronavirus LFD-RPA. Background technique [0002] Porcine deltacoronavirus (PDCoV) is a newly emerged coronavirus that was first identified in Hong Kong, China, in 2012. Since then, PDCoV has been reported successively around the world, and its infection can cause clinical symptoms such as diarrhea, vomiting, dehydration, and weight loss in pigs, and severe cases can lead to death of newborn piglets. At present, PDCoV is often co-infected with other viral diarrhea pathogens, and the clinical symptoms are also very similar, which increases the difficulty of PDCoV diagnosis. At present, there is no simple and reliable diagnostic kit for PDCOV detection. It is a serious challenge for the pig industry. Contents of the invention [0003] The object of the present invention is a primer probe set and a kit for rapid dete...

AI Technical Summary

Problems solved by technology

At present, PDCoV is often co-infected with other viral diarrhea pathogens, and the clinical symptoms are also very similar, which increases the difficulty of PDCoV diagnosis. Currently, there is no simple and reliable diagnostic kit for PDCOV detection. A serious challenge for the swine industry

Images