Rapid, simple and convenient deafness gene detection method and kit

A kit and primer combination technology is applied in the field of biomedicine to achieve the effects of saving detection time, high precision and short detection time period
CN113106152AInactive Publication Date: 2021-07-13北京尔惠基因科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
北京尔惠基因科技有限公司
Publication Date
2021-07-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a rapid, simple and convenient deafness gene detection method and a kit. The kit comprises a primer combination for detecting deafness gene mutation, the primer combination is composed of G235WF, G235MF, G235WR, SIV72WF, SIV72WR and SIV72MR, and the sequences of the primer combination are as follows: the 5'tail ends of the G235WF, the G235MF, the G235WR, the SIV72WF, the SIV72WR and the SIV72MR are respectively marked with fluorescein FITC, TAMATA, biotin biotin, biotin biotin, fluorescein FAM and DIG. Experiments prove that the kit disclosed by the invention can be used for rapidly, accurately and sensitively detecting mutation conditions of two sites of GJB2: c.235delC and SLC26A4:c.919-2A>G.
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Description

technical field

[0001] The invention relates to a quick and simple deafness gene detection method and kit in the field of biomedicine. Background technique

[0002] Deafness is one of the most common diseases that threaten human health and disability, and it is also the most common disease that causes communication barriers. Deafness is divided into non-synthetic deafness and comprehensive deafness according to different performance characteristics. Studies have shown that at least 50% of congenital deafness is caused by genetic factors, and about 77% of non-synthetic deafness is autosomal recessive. There are many pathogenic factors for deafness. Among all deaf patients, hereditary deafness accounts for about 50%; deafness-causing gene mutations can be found in 70%-80% of hereditary deafness patients.

[0003] According to different ways of inheritance, hereditary deafness is divided into five types: autosomal dominant inheritance (AD), autosomal recessive inheritance (AR)...

Claims

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