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Preparation method of Cu nanometer material and cancer cell detection method

A technology of nano-materials and detection methods, applied in the direction of analysis materials, metal material coating technology, measurement devices, etc., can solve the problems of cardiac pacemaker patients who cannot be used, malignant tumors, leukemia, and expensive CT equipment, etc., to achieve decentralized Good performance, simple preparation and low cost

Inactive Publication Date: 2016-04-13
AFFILIATED YONGCHUAN HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CT, referred to as computerized tomography, can be used to check the whole body lesions, and can clarify the extent of cancer lesion invasion and whether it invades the surrounding organs. However, CT equipment is expensive and expensive, and excessive CT may induce malignant tumors, leukemia and other risks
MRI, referred to as nuclear magnetic resonance imaging, can clearly diagnose deep brain tumors, brainstem tumors, spinal cord tumors, mediastinal tumors, heart tumors, etc. Patients with clips etc. cannot use

Method used

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Experimental program
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Effect test

Embodiment 1

[0024] The preparation method of embodiment 1Cu nanometer material

[0025] The preparation method of the Cu nanomaterial modified by hyaluronic acid of the present invention comprises the following steps:

[0026] Preparation of rod-like ZnO by direct deposition method;

[0027] Ni is plated on ZnO, and it comprises the following steps: ZnO is sensitized in the solution of tin dichloride and hydrochloric acid, puts into the solution of palladium dichloride and hydrochloric acid to activate again, and the activated ZnO is cleaned with deionized water , and then put ZnO into the electroless plating pool containing nickel sulfate, sodium hypophosphite, ammonium chloride, sodium citrate, succinic acid and lactic acid for electroless plating, and finally filter the nickel-plated ZnO with deionized water After washing and drying, nickel-plated ZnO is obtained.

[0028] Coating nickel-plated ZnO with Cu comprises the following steps: moving the prepared nickel-plated ZnO into deio...

Embodiment 2

[0030] Embodiment 2 cancer cell detection method

[0031] Cancer cell detection method of the present invention comprises the following steps:

[0032] a) Fluorescein labeling, dissolving the Cu nanomaterial in deionized water, adding fluorescein isothiocyanate for labeling, the particle size range of the Cu nanomaterial is 40-60nm, hyaluronic acid in the Cu nanomaterial The loading capacity on the surface is 0.4~0.5mmol / g;

[0033] b) Cultivation of cancer cells, wherein the cancer cells are one of lung cancer cells, liver cancer cells, breast cancer cells or gastric cancer cells. 10 5 Each well was inoculated in a well plate, incubated at 30-37°C, 5% CO2 for 24 hours, washed with alkaline phosphate solution, added copper nanomaterial solution labeled with fluorescein isothiocyanate, incubated for 24 hours, washed with alkali Fully wash with alkaline phosphate solution, add 1ml 4% paraformaldehyde solution to each well and fix for 20min, then wash with alkaline phosphate s...

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Abstract

The invention discloses a preparation method of a Cu nanometer material. The preparation method comprises the following steps that bar-shaped ZnO is prepared through a direct deposition method, then ZnO is plated with Ni, nickel-plated ZnO is wrapped by Cu, and finally the Cu-wrapped nickel-plated ZnO nanometer material is modified through hyaluronic acid. A cancer cell detection method comprises the following steps that the Cu nanometer material is subjected to fluorescein marking, then the Cu nanometer material and cancer cells are cultivated together, and then put under a laser microscope to be observed, and the number of the cancer cells is quantitatively detected according to the staining quantity. The Cu nanometer material prepared through the method has the beneficial effects of being easy to prepare, good in biocompatibility, capable of being combined with the cancer cell specific receptor CD44 and the like. According to the method for utilizing the Cu nanometer materials for detecting the cancer cells, only the blood of a patient needs to be collected for in-vitro detection, the cancer cells can be recognized in few samples, and the cancer can be accurately, sensitively and atraumatically diagnosed in the initial stage of illness.

Description

technical field [0001] The invention belongs to the field of nanomaterial preparation and biomedical application, and in particular relates to a preparation method of hyaluronic acid-modified Cu-coated nickel-plated ZnO nanometer material and a method for detecting cancer cells by using the material. Background technique [0002] Cancer is one of the major diseases that threaten human health and life, and its incidence is increasing day by day. At present, a large number of clinical facts have proved that the cure rate of the early stage of cancer is quite high. Therefore, early diagnosis is of great significance to improve the survival rate of cancer patients. There is an urgent need to find an accurate, sensitive, and easy-to-operate early diagnosis method. [0003] At present, cancer detection methods mainly include B-ultrasound, CT, MRI, biopsy and so on. B-ultrasound can continuously display and observe lesions from angles and multiple directions by using section son...

Claims

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Application Information

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IPC IPC(8): B22F1/02C23C18/18C23C18/36G01N33/574
CPCG01N33/574C23C18/18C23C18/36B22F1/07B22F1/17
Inventor 邓怡邓大才
Owner AFFILIATED YONGCHUAN HOSPITAL OF CHONGQING MEDICAL UNIV
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