Confining liquid and application thereof

A technology of blocking liquid and solution, which is applied in the direction of instruments, analytical materials, particles and sedimentation analysis, etc., can solve the problems of perishable, poor sealing effect of blocking liquid, etc., and achieve the effect of not easy to deteriorate, saving experimental cost, and accurate results
CN110646335AInactive Publication Date: 2020-01-03GUANGDONG UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGDONG UNIV OF TECH
Publication Date
2020-01-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the technical field of confining liquid, in particular to confining liquid and application thereof. The invention discloses confining liquid. The confining liquid comprises first confining liquid and second confining liquid; the first confining liquid comprises Twain-20, donkey serum, bovine serum albumin and a phosphate buffer solution; the second confining liquid comprises Twain-20, donkey serum, bovine serum albumin, a phosphate buffer solution and polyethylene glycol octyl phenyl ether. According to the confining liquid provided by the invention, the condition thatFcR on a cell surface can be non-specifically combined with the Fc segment of a fluorescein coupling antibody effectively be reduced, and the confining effect is good, so that the result of flow cytometry is more accurate, and the confining liquid is unlikely to go bad.
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Description

technical field

[0001] The invention relates to the technical field of blocking liquid, in particular to a blocking liquid and its application. Background technique

[0002] Flow cytometry is an analytical technique that can perform multiparameter, rapid quantitative analysis of single cells or other biological particles. It can analyze tens of thousands of cells at high speed, and can measure multiple parameters from one cell at the same time. It has the advantages of high speed, high precision and good accuracy. It is one of the most advanced cell quantitative analysis technologies in the contemporary era. Quantitative analysis of cardiomyocytes by flow cytometry can obtain data information on the differentiation of human induced pluripotent stem cells (hiPSCs) into cardiomyocytes, so as to understand the differentiation of cardiomyocytes and provide data support for the follow-up work of culturing cardiomyocytes .

[0003] Most of the fluorescein-conjugated antibodies t...

Claims

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