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Method for rapidly detecting protein in food by using indigo dye

A technology of indigo dye and detection method, which is used in material excitation analysis, fluorescence/phosphorescence, etc., to achieve accurate, convenient and rapid detection, and to improve sensitivity and selectivity.

Inactive Publication Date: 2013-12-11
GUILIN UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

The study found that the research on indigo mainly focused on the determination of the content in the drug and its dyeing ability, but there were no reports on the use of indigo's resonance light scattering signal to determine the protein in food

Method used

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  • Method for rapidly detecting protein in food by using indigo dye
  • Method for rapidly detecting protein in food by using indigo dye
  • Method for rapidly detecting protein in food by using indigo dye

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Embodiment Construction

[0014] 1. Detection method:

[0015] Add 1.00 ml of 1.0×10 to each of twelve 10 ml colorimetric tubes -4 mol / L indigo solution, then add 20, 60, 100, 140, 180, 220, 260, 340, 420, 500, 580, 600 microliters of 1.0×10 -3 mol / liter of bovine serum albumin solution, and then use pH=4.5, concentration of 0.05 mol / liter of acetic acid-sodium acetate buffer solution to set the volume to the scale, and react for 3 minutes to carry out resonance light intensity detection with a fluorescence photometer, so that The excitation wavelength is the same as the emission wavelength, and the excitation and emission slit widths are both 5 nm.

[0016] 2. Drawing of standard working curve:

[0017] Add 1.00 ml of 1.0×10 to each of twelve 10 ml colorimetric tubes -4 mol / L indigo solution, then add 20, 60, 100, 140, 180, 220, 260, 340, 420, 500, 580, 600 microliters of 1.0×10 -3 mol / liter bovine serum albumin solution, and then use pH=4.5 and acetic acid-sodium acetate buffer solution with a co...

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Abstract

The invention discloses a method for rapidly detecting protein in food by using indigo dye. An aggregate is formed by indigo and bovine serum albumin through electrostatic interaction and hydrophobic interaction, and the resonance light scattering intensity of indigo is correspondingly enhanced, so as to establish the method for measuring the protein content in food. A resonance light scattering signal added value of indigo has a good linear relationship with the concentration of the bovine serum albumin to be measured when the concentration is within 2*10<-8> to 6*10<-7>mol / L (1.3*10<-3> to 3.9*10<-2>g / L), and the detection limit of the method is 6*10<-9>mol / L (3.9*10<-4>g / L). By utilizing the method, the defects that the conventional technique is low in sensitivity, narrow in application range and the like in detection are overcome, the sensitivity and the selectivity are improved, and the low-concentration protein in food can be detected conveniently and rapidly.

Description

technical field [0001] The invention relates to a method for rapidly detecting trace protein in food by using indigo dye and resonance light scattering technology. Background technique [0002] Protein is the material basis of life. It plays a vital role in participating in the reaction of the human body, regulating metabolism, resisting the invasion of foreign substances, and controlling genetic information. The quantitative detection of protein is an important research topic in life sciences. . Therefore, it is of great significance to seek a method for detecting protein in food with high sensitivity and high selectivity. At present, the methods for protein determination mainly include fluorescence analysis, chromatography, chemiluminescence, resonance light scattering and so on. Among them, the application of resonance light scattering technology to the analysis of biological macromolecules has the characteristics of simplicity and high sensitivity, which provides a new...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 陶慧林徐铭泽张庆军黎舒怀廖秀芬孙超
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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