Method for detecting gibberellin in food using cyclodextrin and fluorescence resonance energy transfer technology
A fluorescence resonance energy, technical detection technology, applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., to achieve accurate, convenient and fast detection, and improve sensitivity and selectivity.
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[0015] 1. Detection method:
[0016] Add 100 microliters of 1.0×10 to each of ten 10ml colorimetric tubes -5 mol / L riboflavin solution, 120 microliters 1.0×10 -4 mol / liter of butylrhodamine B solution, 1 ml of 1.0×10 -3 mol / L of β-cyclodextrin solution and 20 microliters of 0.1 g / L polyvinyl alcohol solution, and then add 40, 120, 200, 280, 360, 440, 520 , 600, 680, and 760 ng / mL gibberellin solutions, respectively, with disodium hydrogen phosphate (0.2 mol / L)-citric acid (0.1 mol / L) buffer solution of pH=6.6 to adjust the volume to the mark, and react 3 Minutes later, the fluorescence intensity was detected with a RF-5301PC fluorescence photometer, the excitation wavelength was 360 nm, and the excitation and emission slit widths were both 5 nm.
[0017] 2. Drawing of working curve:
[0018] Add 100 microliters of 1.0×10 to each of ten 10ml colorimetric tubes -5 mol / L riboflavin solution, 120 microliters 1.0×10 -4 mol / liter of butylrhodamine B solution, 1 ml of 1.0×10 -...
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