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Method for judging sex of trachinotus ovatus at early stage

A technology of ovoid pomfret and gender, applied in fish farming, application, climate change adaptation, etc., can solve the difficulties in the establishment of induced labor and selective breeding families, unfavorable development of ovate pomfret breeding industry, and technical requirements for testing personnel For advanced problems, it achieves the effect of easy judgment method, obvious difference of gonad characteristics, and improvement of observation efficiency

Active Publication Date: 2014-08-13
GUANGXI ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Benefits of technology

In this patented technology, it provides a way to quickly identify poultry from other species based on its DNA profile without requiring laborious procedures like histopathologic examining. Additionally, there have been developed techniques that allow scientists to study young birds more accurately than older ones while also reducing costs associated with these tests. These improvements make it easier to cram seedling crops faster and cheaper.

Problems solved by technology

There currently exist various techniques related to sorting out gametes from piglets based on their physical properties such as size, color texture, odor intensity, etc., including genetics analysis technology. These technologies involve analyzing DNA sequences specific patterns associated with certain traits like race ability, fertility, and necrosis/dwarancy among young swallowings. They also help researcher discover new genome types through genomics studies.

Method used

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  • Method for judging sex of trachinotus ovatus at early stage

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Experimental program
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Effect test

Embodiment 1

[0026] 1. Take the first-year-old oval pomfret, cut off the left body wall to expose the internal organs.

[0027] 2. Find the intestinal tract and the left and right gonads from the cloaca toward the abdominal cavity. The gonads are located on both sides of the back of the swim bladder. The gonads are long and thin, milky white, and small in size.

[0028] 3. Carefully peel off other visceral tissues other than the gonads, take out the complete unilateral gonads, cut them transversely from the middle, observe with the naked eye and under an anatomical microscope, they are all solid structures.

[0029] 4. Determine that the gonad is the testis, and the oval pomfret is male.

[0030] To verify the accuracy of this method, the following slice experiments were performed:

[0031] The gonads on the other side were taken out and fixed in 4% PFA solution for 24 hours. After dehydration with 70%-100% gradient ethanol, routine paraffin sections and HE staining were performed. The m...

Embodiment 2

[0034] 1. Take the first-year-old oval pomfret, cut off the left body wall to expose the internal organs.

[0035] 2. Find the intestinal tract and the left and right gonads from the cloaca towards the abdominal cavity. Observe in situ that the gonads are located on both sides of the back of the swim bladder, close to the body wall and kidneys.

[0036] 3. Carefully peel off other visceral tissues other than the gonads, take out the complete unilateral gonads, cut them transversely from the middle, observe with the naked eye and under an anatomical microscope, they are all hollow structures, and granular oocytes attached to the inner wall of the egg cavity can be seen under the microscope cell.

[0037] 4. It is determined that the gonad is an ovary, and the oval pomfret is a female.

[0038] To verify the accuracy of this method, the following slice experiments were performed:

[0039] The gonads on the other side were taken out and fixed in 4% PFA solution for 24 hours. Af...

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Abstract

The invention discloses a method for judging the sex of trachinotus ovatus at an early stage. Young trachinotus ovatus is selected, the left body wall is cut off, sex gland is found out, characteristics of the sex gland are observed in situ, the sex gland is taken out and dissected, the characteristics of the dissection face of the sex gland are observed, and the sex of the trachinotus ovatus is judged by analyzing the characteristics of the sex gland According to the method, the instrument is simple, operation is rapid, the result is accurate, toxic chemical agents are not used, the method for judging the sex of the trachinotus ovatus at the early stage is convenient to carry out efficient, and reliable materials can be provided for molecular marker research for further searching for sex specialty of the trachinotus ovatus.

Description

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Claims

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Application Information

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Owner GUANGXI ACADEMY OF FISHERY SCI
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